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| 秋水仙素诱导大葱多倍体的研究 | |
| 引用本文： | 董飞,陈运起,刘世琦,高莉敏,王传增,陈伟. 秋水仙素诱导大葱多倍体的研究[J]. 园艺学报, 2011, 38(12): 2381-2386 |
| 作者姓名： | 董飞 陈运起 刘世琦 高莉敏 王传增 陈伟 |
| 作者单位： | 1. 山东农业大学园艺科学与工程学院,山东泰安,271018 2. 山东省农业科学院蔬菜研究所／山东省设施蔬菜生物学重点实验室／国家蔬菜改良中心山东分中心,济南,250100 |
| 基金项目： | 国家公益性行业(农业)科研专项(200903018); 山东省良种产业化项目 |
| 摘 要： | 以章丘大葱种子为材料，研究了秋水仙素处理浓度和时间对大葱体细胞染色体加倍的效果，并利用形态观察，根尖细胞染色体计数及流式细胞仪等方法进行倍性鉴定。结果表明，0.8%秋水仙素处理48 h的诱变效果最佳。诱变植株存在明显“玻璃化”现象，生根困难，移栽后不能成活。通过组织培养去“玻璃化”，获得正常生长的四倍体植株。四倍体植株的细胞染色体数2n = 4x = 32，植株多倍体形态特征明显，植株生长健壮，叶色浓绿，叶面气孔变大，气孔密度减少；株高、葱白长度等与二倍体植株相比极显著增加。 |
| 关 键 词： | 大葱 秋水仙素 四倍体诱导 鉴定 |
| 收稿时间： | 2011-09-22 |
| 修稿时间： | 2011-11-29 |
Colchicines Induced Polyploid Plants and Identification in Welsh Onion |
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| DONG Fei,CHEN Yun-qi,LIU Shi-qi,GAO Li-min,WANG Chuan-zeng, CHEN Wei. Colchicines Induced Polyploid Plants and Identification in Welsh Onion[J]. Acta Horticulturae Sinica, 2011, 38(12): 2381-2386 | |
| Authors: | DONG Fei CHEN Yun-qi LIU Shi-qi GAO Li-min WANG Chuan-zeng CHEN Wei |
| Affiliation: | DONG Fei1,CHEN Yun-qi2,LIU Shi-qi1,GAO Li-min2,WANG Chuan-zeng1, CHEN Wei2 |
| Abstract: | The welsh onion seeds were treated with colchicines at different concentration and treatment time duration,and the ploidy level was identified by morphological trait,chromosome number and flow cytometry techniques.The results showed that the best effect was obtained by the treatment of 0.8% colchicines with 48 hours.The mutated plants we got were vitreous seedlings,they could not survive after transplanting.By tissue culture we obtained tetraploids.The choromosome number of tetraploids was 2n = 4x = 32,the ... |
| Keywords: | welsh onion colchicine tetraploid induction identification |
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