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| 内标校准液相色谱荧光法测定食用油中苯并(a)芘 | |
| 引用本文： | 王秀嫔,§,李培武,§,王 琳,张 文,张 奇,甘冬生. 内标校准液相色谱荧光法测定食用油中苯并(a)芘[J]. 中国油料作物学报, 2011, 33(6): 603-608 |
| 作者姓名： | 王秀嫔 § 李培武 § 王 琳 张 文 张 奇 甘冬生 |
| 作者单位： | 1. 中国农业科学院油料作物研究所，湖北 武汉 430062；2. 农业部油料作物生物学重点开放实验室，湖北 武汉 430062；3. 农业部油料及制品质量监督检验测试中心，湖北 武汉 430062) |
| 基金项目： | 国家油菜现代产业技术体系(cars-13); 国家自然科学基金(31000877); 农业部948项目(2010-G1,2011-G5); 国家科技支撑计划(2010BAK08B03,2012BAB19B09); 中国农业科学院油料作物研究所所长基金 |
| 摘 要： | 采用内标苯并（b）屈校正，建立了正相固相净化－反相液相色谱－荧光分光光度法测定食用油中苯并(a)芘的方法。正相Plus Silica固相萃取柱作为净化柱，反相C18柱作为分离柱。以苯并（b）屈作为内标，测定了苯并(a)芘的校正因子。使用本方法，苯并(a)芘在液态食用油和固态食用油中的检出限分别为0.01和0.03μg/kg。苯并(a)芘在不同食用油回收率在83%～108%之间，方法的日内精密度和日间精密度分别小于7.7%和13.2%。 |
| 关 键 词： | 苯并(a)芘 苯并(b)屈 内标 正相固相净化 HPLC-FLD |
Determination of benzo[a]pyrene in edible oils by liquid chromatographyfluorescence detection with benzo[b]chrysene as internal standard |
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| WANG Xiu-pin,,,LI Pei-wu ,,WANG Lin,ZHANG Wen,ZHANG Qi,GAN Dong-sheng. Determination of benzo[a]pyrene in edible oils by liquid chromatographyfluorescence detection with benzo[b]chrysene as internal standard[J]. Chinese Journal of Oil Crop Sciences, 2011, 33(6): 603-608 | |
| Authors: | WANG Xiu-pin LI Pei-wu WANG Lin ZHANG Wen ZHANG Qi GAN Dong-sheng |
| Affiliation: | WANG Xiu-pin1,2,3,LI Pei-wu 1,4,WANG Lin1,ZHANG Wen1,ZHANG Qi1,GAN Dong-sheng1,3 (1.Oil Crops Research Institute of Chinese Academy of Agricultural Sciences,Wuhan 430062,China,2.Key Laboratory of Oil Crop Biology and Genetic Improvement of the Ministry of Agriculture,3.Key Laboratory of Biotoxins,MOA,4.Quality Inspection and Test Center for Oilseeds Products,China) |
| Abstract: | The normal-phase solid phase cleaning-reversed high performance liquid chromatography-fluorescence detection was applied for the determination of the benzo[a]pyrene(BaP) in edible oils.The edible oil samples were cleansed with a Plus Silica solid phase extraction cartridge.The separation was carried out on a C18 column with gradient elution.With benzo[b]chrysene(BbC) as the internal standard,the relative correction factor(RCF) of BaP was determined by HPLC-FLD;the BaP was determined by using BbC as the sing... |
| Keywords: | Benzo[a]pyrene Benzo[b]chrysene Internal standard Normal-phase solid phase cleaning HPLC-FLD |
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