| 羽衣甘蓝ARC1的基因分离、表达及与SRK相互作用的分析 |
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| 引用本文： | 蓝兴国,杨佳,赵昕,李玉花.羽衣甘蓝ARC1的基因分离、表达及与SRK相互作用的分析[J].园艺学报,2011,38(12):2342-2348. |
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| 作者姓名： | 蓝兴国 杨佳 赵昕 李玉花 |
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| 作者单位： | 东北林业大学生命科学学院,哈尔滨,150040 |
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| 基金项目： | 国家自然科学基金项目(30900115,31070275); 中央高校基本科研业务费专项基金项目(DL09BA08) |
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| 摘 要： | 以羽衣甘蓝（Brassica oleracea var. acephala）自交不亲和系（S13-b S13-b）为试验材料，利用RT-PCR的方法分离其ARC1基因cDNA序列（命名为BoARC1，GenBank登录号为EU344909）。BoARC1 cDNA序列中含有1个1 992 bp的开放读码框（ORF），编码1个含有663个氨基酸的蛋白质。羽衣甘蓝BoARC1与油菜BnARC1的氨基酸序列具有94%的一致性；Southern杂交结果显示BoARC1在基因组中只存在单一拷贝；Northern杂交结果显示BoARC1特异性地在柱头组织中表达；在酵母双杂交试验分析中，BoARC1能够与SRK3、SRK 13-b和SRK910的胞内激酶域发生相互作用。
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| 关 键 词： | 羽衣甘蓝 自交不亲和 ARC1 相互作用 |
| 收稿时间： | 2011-5-10 |
| 修稿时间： | 2011-10-25 |
Isolation,Expression of ARC1 from Ornamental Kale and Interaction Analysis Between ARC1 and SRK |
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| LAN Xing-guo,YANG Jia,ZHAO Xin, LI Yu-hua.Isolation,Expression of ARC1 from Ornamental Kale and Interaction Analysis Between ARC1 and SRK[J].Acta Horticulturae Sinica,2011,38(12):2342-2348. |
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| Authors: | LAN Xing-guo YANG Jia ZHAO Xin LI Yu-hua |
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| Institution: | LAN Xing-guo,YANG Jia,ZHAO Xin,and LI Yu-hua(College of Life Sciences,Northeast Forestry University,Harbin 150040,China) |
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| Abstract: | The ARC1 cDNA sequence was isolated from stigma of ornamental kal(e Brassica oleracea var.acephala)S13-bS13-b homozygotes using RT-PCR techniques,named BoARC1(GenBank accession No.EU344909).The BoARC1 cDNA sequence had an ORF of 1 992 bp,encoding a 663-aa protein.The deduced amino acid sequence of isolated BoARC1 shared 94% identity with Brassica napus ARC1.Southern blot analysis showed that there was a single copy of BoARC1 gene in genome.Northern blot analysis showed that BoARC1 mRNA only expressed in the... |
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| Keywords: | Brassica oleracea var acephala self-incompatibility ARC1 interaction |
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