茶树秋梢芽、叶间几种同工酶的比较研究

秋梢从芽到第五叶,表现出3种酶谱共有15条过氧化物酶同工酶带表达。酶带PX2为1至4叶的特异性酶带,PX13带的活性及总活性,随新梢的老化而递增。PX15带的活性在2和4叶中出现两个最高峰值。芽的过氧化物酶活性,与各叶片比较,均达极显著水平。多酚氧化酶在芽叶中表达出6条酶带、两种酶谱。多酚氧化酶活性以芽和第三叶最低,而芽又比各叶片显著地低。在各部位间活性变化较大的酶带有 PL1、PL3、PL6等。秋梢中的酯酶同工酶谱共有9条酶带,各部位的酶谱均相同。但是酶带 ES13、ES14的活性表现出随叶片老化而递减

Comparative Studies on Isozymograms and Activities of Some Enzymes in Organs of Tea Shoot in Autumn Season

Some studies on the isozymes of peroxidase,polyphenol oxidase,esterase and theactivities of peroxidase,polyphenol oxidase in six parts(including one bud and fiveleaves)of growing tea shoot in autumn season were conducted.The results are asfollows:15 bands of peroxidase,6 bands of polyphenol oxidase,and 9 bands of esterasewere detected.There were three kinds of peroxidase isozymograms,two kinds ofpolyphenol oxidase isozymograms and the esterase isozymogram showed no differenceamong the bud and leaves.The band PX_2 of peroxidase was found as the marker bandin the first leaf to the fourth leaf.The sum activity of peroxidase and it's band PX_(13)increased as the organ changedfrom bud to the fifth leaf and the activity of band PX_(15)showed two highest peaks inthe second leaf and the fifth leaf.The activities of polyphenol oxidase in bud and thethird leaf were lower than those in other leaves.The bands in the polyphenol oxidaseisozymogram showing difference in activity among the bud and leaves were PL_1,PL_3and PL_8.In the isozymogram of esterase the activities of bands ES_(13)ES_(14)significantlydecreased as the organsochanged from bud to the fifth leaf,showing opposite to thatof peroxidase.These results indicated that the peroxidase isozymograms,polyphenol oxidasewere correlative with the development of tea shoot.And some active bands such asPX_2,PX_(13),PX_(15),PL_1,PL_3,PL_8,ES_(13),ES_(14)of the three kinds of the enzymesindecated there were differences in organs in metabolize and ageing of the leavse of teashoot.