降解菌株P-2生物学特性及其降解性能研究

为降解土壤中农药残留,解决农副产品农药超标问题,采用富集培养法分离筛选出1株能够降解多菌灵的菌株P-2,研究初始pH、培养温度、接种量、外加碳源、氮源对其生长量和降解特性的影响。结果表明:该菌株能以多菌灵为碳源生长,在基础培养基中培养5d时对100mg.L-1的多菌灵降解率达60.6%,而另外加入氮源蛋白胨,可提高降解率达91%。降解多菌灵的适宜条件为温度25～40℃、pH 5.1～8.1,且降解率与菌体生长量呈正相关关系。

Biological characteristics and degradation performance of a degrading strain

To degrade pesticide residues in soil and solve the problem of excessive pesticide residues in agricultural byproducts,a bacterium was screened and isolated from the carbendazim polluted soil.The effect of some factors on its growth biodegradation characteristics in vitro were studied,including initial pH,culture temperature,inoculum size,extra carbon source,nitrogen source.The results showed that this strain could grow by using carbendazim as the carbon source.It could degrade 60.6% of 100 mg·L-1 carbendazim in mineral culture medium in 5 d,but could degrade 91% by adding peptone as nitrogen source.The optimal pH and culture temperature for carbendazim biodegradation were 5.1-8.1 and 25-40 ℃ respectively.The biodegradation speed was related positively to the biomass yield.