环境因子对西花蓟马室内生物测定结果的影响

采用Munger cell法,就室内测定多杀菌素对西花蓟马 Frankliniella occidentalis 生物活性实验中需要控制的温度、相对湿度、滴水量、光周期、光照度和叶片6个环境条件对测定结果的影响进行了研究。发现:在25 ~30 ℃、相对湿度50% ~70%、光周期(L:D)为16 h:8 h~24 h:0 h、光照度6 500~13 000 lx这一测定条件范围内,采用月季、西葫芦、萝卜、芸豆、苹果、芹菜、菠菜叶片进行生物测定时结果较稳定;叶片纤毛数量与多杀菌素对西花蓟马的LC50值成负相关,相关系数为0.919;叶片纤毛的长度则与测定结果无相关性。

Effect of environmental factors on the results of laboratory bioassay of Frankliniella occidentalis

The effect of environmental factors such as temperature,relative humidity(RH),water quantity,photoperiod,illuminance and leaf on the results of laboratory bioassay of spinosad to Frankliniella occidentalis(Pergande) was studied using method of Munger cell.The results showed that during the bioassay conditions ranges of temperature 25-30 ℃,RH 50%-70%,water quantity 0.5-1.5 mL,photoperiod 16 h ∶8 h-24 h ∶0 h(L ∶D),illuminance 6 500-13 000 lx and with the leaves of Rosa chinensis,Cucurbita pepo,Raphanus sativus,Phaseolus vulgaris,Malus pumila mill,Apiumgraveolens,Spinacia oleracea,the laboratory bioassay results preserve stationary.The leaf cilium density has negative correlation with the bioassay result of spinosad to F.occidentalis,and the related coefficient was 0.919;the length of leaf cilium has no correlation with the bioassay result.