不同裂果类型番茄成熟过程果皮组织衰老研究

针对抗裂(17922)、环裂(16750)、射裂(16620)3个番茄品种在成熟过程中(绿熟期、转色期和红熟期)不同部位(果肩、果腹和果顶)果皮,研究其抗氧化酶活性、抗氧化物质、活性氧含量及膜质过氧化程度。结果表明,16750和16620在红熟期果肩活性氧含量、SOD和CAT活性均显著高于果腹和果顶,在转色期和红熟期果肩膜质过氧化程度和相对电导率均显著高于果腹和果顶,且均显著高于17922。16750和16620,GR和AsA含量在成熟过程中均显著低于17922,而POD活性在成熟过程中显著高于17922,APX在转色期果肩处显著高于17922。说明16750和16620在转色期和红熟期,果肩处果皮抗氧化酶活性和抗氧化物质含量较低,活性氧代谢失调,膜质过氧化程度较高,衰老加剧。研究为进一步探讨果实裂果机制提供理论基础。

Study on pericarp tissue senescence of different fruit cracking types of tomato during ripening process

In this paper, pericarp's antioxidant enzyme activity, antioxidant substance, reactive oxygen species(ROS) content and membrane peroxidation degree of cracking-resistant(17922), ringcracking(16750) and radial-cracking(16620) in different parts(fruit shoulder, fruit belly, and fruit top)during the ripening process(green stage, turn stage, and red ripe stage) were studied. The results showed that the active oxygen content, SOD and CAT activities of the 16750 and 16620 in fruit shoulders were significantly higher than those of the fruit belly and fruit top during the red ripening period. And the membrane peroxidation degree and relative conductivity of fruit shoulder during turn stage and red ripe stage were significantly higher than those of fruit belly and fruit top, at the same time,they were significant higher than those of 17922. The GR and AsA contents of 16750 and 16620 were significantly lower than 17922 during the whole ripening process, and the POD activity was significantly higher than 17922, while the APX was significantly higher than that of 17922 in the turn stage. It indicated that during the turn stage and red ripe stage, the pericarp at fruit shoulder had the low antioxidant enzyme activity and antioxidant content, the disordered reactive oxygen species metabolism,at the same time, the degree of membrane peroxidation became high, all these factors had led to an increase in aging. This study provided a theoretical basis for further exploring the mechanism of cracking.