Simple sequence repeats as useful resources to study transcribed genes of cotton |
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| Authors: | Sukumar Saha Mehmet Karaca Johnie N Jenkins Allan E Zipf O Umesh K Reddy Ramesh V Kantety |
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| Institution: | (1) USDA-ARS, Crop Science Research Laboratory, P.O. Box 5367, Mississippi State, MS, 39762, U.S.A;(2) Department of Field Crops, Faculty of Agriculture, Akdeniz University, 07059 Antalya, Turkey;(3) Biotechnology Center, Alcorn State University, MS, 39096, U.S.A;(4) Department of Plant Breeding, Cornell University, Ithaca, NY, 14853, U.S.A |
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| Abstract: | Microsatellites or Simple Sequence Repeats(SSRs) are informative molecular genetic markers in many crop species. SSRs are
PCR-based, highly polymorphic, abundant, widely distributed throughout the genome and inherited in a co-dominant manner in
most cases. Here we describe the presence of SSRs in cDNAs of cotton. Thirty one SSR primer pairs of 220 (∼14%) tested led
to PCR amplification of discrete fragments using cotton leaf cDNA as template. Sequence analysis showed 25% of 24randomly
selected cDNA clones amplified with different SSR primer pairs contained repeat motifs. We further showed that sequences from
the SSR-containing cDNAs were conserved across G. barbadense and G. hirsutum, revealing the importance of the SSR markers for comparative mapping of transcribed genes. Data mining for plant SSR-ESTs
from the publicly available databases identified SSRs motifs in many plant species,including cotton, in a range of 1.1 to4.8%
of the submitted ESTs for a given species.
This revised version was published online in August 2006 with corrections to the Cover Date. |
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| Keywords: | Cotton differential display EST SSR |
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