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Segregation and linkage analysis of DNA markers in microspore derived and F2 populations of oilseed rape (Brassica napus L.)
Authors:P K Tanhuanpää  J P Vilkki  H J Vilkki
Institution:(1) Institute of Plant Breeding, Agricultural Research Centre of Finland, FIN-31600 Jokioinen, Finland
Abstract:Summary The segregation of RFLP and RAPD markers was compared in two oilseed rape (Brassica napus L.) breeding populations from the cross lsquoTopasrsquo x R4, the latter being a low linolenic mutation line. A total progeny of 68 F2 and 40 microspore derived plants were studied with 25 markers. The results indicated a significant excess of lsquoTopasrsquo alleles at five RAPD loci in the microspore derived population. This suggests that genomic regions which probably affect microspore culture ability do not have identical distribution in the two population types.
Keywords:Brassica napus  microspore derived population  RAPD  RFLP
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