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| 小麦抗叶锈基因Lr24、Lr35的STS、SCAR标记在近等基因系(NILs)上的特异性验证 | |
| 引用本文： | 刘莉,陈云芳,刘华梁,杨文香,张汀,刘大群. 小麦抗叶锈基因Lr24、Lr35的STS、SCAR标记在近等基因系(NILs)上的特异性验证[J]. 分子植物育种, 2008, 6(3): 471-474 |
| 作者姓名： | 刘莉 陈云芳 刘华梁 杨文香 张汀 刘大群 |
| 作者单位： | 河北省植保植检站,石家庄,050011;苏州出入境检验检疫局,苏州,215126;河北农业大学植物保护学院植物病理系,河北省农作物病虫害生物防治工程技术研究中心,保定,071001 |
| 基金项目： | 河北省社会科学基金 , 国家973前期项目 |
| 摘 要： | 用一套分别含有不同抗叶锈基因的53个以Thatcher为遗传背景的近等基因系（near-isogeniclines,NILs）对已报道的分别与抗叶锈基因Lr24和Lr35连锁的STS、SCAR进行特异性验证。结果对于与Lr24连锁的STS标记,在53个NILs中只在TcLr24亲本中扩增出片段大小与报道相同的310bp的条带,在TcLr35中也扩增出了一条片段,但片段大小不同于310bp约为270bp。对于与Lr35连锁的SCAR标记,只在TcLr35亲本中扩增出片段大小为900bp的条带,与报道片段大小一致。验证结果表明与抗病基因Lr24和Lr35连锁的STS、SCAR分子标记在NILs中特异性都较好,进一步证明了这两个分子标记可方便地用于小麦抗叶锈基因Lr24、Lr35的分子标记辅助选择育种。 |
| 关 键 词： | 小麦叶锈 抗叶锈病基因 STS分子标记 SCAR分子标记 近等基因系 |
Verification of STS Molecular Markers for Leaf Rust Resistance Genes Lr24 and Lr35 in Near-isogenic Lines of Wheat cv.Thatcher |
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| Liu Li,Chen Yunfang,Liu Hualiang,Yang Wenxiang,Zhang Ting,Liu Daqun. Verification of STS Molecular Markers for Leaf Rust Resistance Genes Lr24 and Lr35 in Near-isogenic Lines of Wheat cv.Thatcher[J]. Molecular Plant Breeding, 2008, 6(3): 471-474 | |
| Authors: | Liu Li Chen Yunfang Liu Hualiang Yang Wenxiang Zhang Ting Liu Daqun |
| Affiliation: | Liu Li 1 Chen Yunfang 2 Liu Hualiang 3 Yang Wenxiang 3 Zhang Ting 3 Liu Daqun 31 Hebei Station of Plants Proteciont , Plants Quarantine,Shijiazhuang,050011,2 SuZhou Entry-Exit Inspection , Quarantine Bureau,Suzhou,215126,3 Department of Plant Pathology,College of Plant Protection,Hebei Agricultural University/ Biological Control Center of Plant Diseases , Pests of Hebei Province,Baoding,071001 |
| Abstract: | Sequence tagged site (STS) markers for Lr24 and Sequence characterized amplified regions (SCAR) marker for Lr35 against Puccinia triticina had been used to screen a set of near-isogenic lines-(NILs) of wheat cv.- Thatcher containing in total 53 different Lr genes and their alleles. Polymerase chain reaction (PCR) analysis was carried out using STS and SCAR primers specific for the leaf rust resistance genes Lr24, Lr35. The amplification product of the STSLr35-Sr39 marker for the Lr35 resistance gene was det... |
| Keywords: | Wheat leaf rust Resistance genes STS molecular marker SCAR molecular marker Near-isogenic lines |
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