|
|||||
|
|
Overproduction of reactive oxygen species involved in the pathogenicity of Fusarium in potato tubers |
|
| Institution: | 1. College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, PR China;2. Gansu Provincial Key Laboratory of Aridland Crop Science/Gansu Key Laboratory of Crop Improvement & Germplasm Enhancement, Lanzhou 730070, PR China;1. Laboratory of Food Chemistry and Toxicology, Faculty of Pharmacy, University of Valencia, Av. Vicent Andrés Estellés s/n, 46100 Burjassot, Spain;2. Department of Analytical Chemistry, Faculty of Pharmacy, University of Valencia, Av. Vicent Andrés Estellés s/n, 46100 Burjassot, Spain;1. Department of Microbial and Molecular Systems, Faculty of Engineering Technology, KU Leuven Bruges Campus, Spoorwegstraat 12, B-8200, Brugge, Belgium;2. Department of Microbial and Molecular Systems, Faculty of Bioscience Engineering, KU Leuven, Celestijnenlaan 200F, B-3000, Leuven, Belgium;3. Prayon NV, Gansbroekstraat 31, B-2870, Ruisbroek, Belgium;4. Department of Chemical Engineering, Faculty of Engineering Technology, KU Leuven Ghent Technology Campus, Gebroeders De Smetstraat 1, B-9000, Gent, Belgium;1. Department of Pediatrics, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung, Taiwan;2. Department of Pharmacology, School of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan;3. Department of Pathology, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung, Taiwan |
| Abstract: | Differences in virulence between Fusarium sulphureum and Fusarium sambucinum were compared. Changes in reactive oxygen species production and metabolism in inoculated slices of potato tubers were also compared. The result showed that Fusarium infection induced significant production of ROS, lipid peroxidation and loss of cell membrane integrity, but low activity of superoxide dismutase (SOD) and ascorbate peroxidase (APX). Compared to F. sambucinum, F. sulphureum led larger lesion diameters on potato tubers and slices. It resulted in more superoxide anion () and earlier peak of hydrogen peroxide (H2O2), but lower activity of catalase (CAT) and APX, and accompanied with higher malondialdehyde (MDA) content and lower cell membrane integrity. These findings suggested that overproduction of ROS involved in the pathogenicity of Fusarium in potato tubers. |
| Keywords: | Potato Infection Reactive oxygen species APX"} {"#name":"keyword" "$":{"id":"kwrd0035"} "$$":[{"#name":"text" "_":"ascorbate peroxidase CAT"} {"#name":"keyword" "$":{"id":"kwrd0045"} "$$":[{"#name":"text" "_":"catalase FW"} {"#name":"keyword" "$":{"id":"kwrd0055"} "$$":[{"#name":"text" "_":"fresh weight GR"} {"#name":"keyword" "$":{"id":"kwrd0065"} "$$":[{"#name":"text" "_":"glutathione reductase hydrogen peroxide MDA"} {"#name":"keyword" "$":{"id":"kwrd0085"} "$$":[{"#name":"text" "_":"malondialdehyde NOX"} {"#name":"keyword" "$":{"id":"kwrd0095"} "$$":[{"#name":"text" "_":"NADPH oxidase POD"} {"#name":"keyword" "$":{"id":"kwrd0105"} "$$":[{"#name":"text" "_":"peroxidase ROS"} {"#name":"keyword" "$":{"id":"kwrd0115"} "$$":[{"#name":"text" "_":"reactive oxygen species RH"} {"#name":"keyword" "$":{"id":"kwrd0125"} "$$":[{"#name":"text" "_":"relative humidity superoxide anion SOD"} {"#name":"keyword" "$":{"id":"kwrd0145"} "$$":[{"#name":"text" "_":"superoxide dismutase |
| 本文献已被 ScienceDirect 等数据库收录！ | |