Identification of allele specific AFLP markers linked with bacterial wilt [Ralstonia solanacearum (Smith) Yabuuchi et al.] resistance in hot peppers (Capsicum annuum L.) |
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Institution: | 1. Centre for Plant Biotechnology and Molecular Biology, Kerala Agricultural University, Thrissur, Kerala 680 656, India;2. Department of Olericulture, College of Horticulture, Kerala Agricultural University, Thrissur, Kerala 680 656, India;3. Department of Agricultural Microbiology, College of Horticulture, Kerala Agricultural University, Thrissur, Kerala 680 656, India;1. Department of Chemistry, Pusan National University, Pusan 46241, Republic of Korea;2. Department of Chemistry, Chonbuk National University, Jeonju 54896, Republic of Korea;1. Modern Research Center for Traditional Chinese Medicine, School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100029, China;2. Department of Medicinal Chemistry, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China;3. Alashan Mongolian Hospital, Alxa Left Banner, Inner Mongolia 750306, China |
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Abstract: | Even though the bacterial wilt is identified as the most destructive disease in hot peppers world-wide, robust molecular markers that facilitate marker assisted selection are absent till date. Kerala Agricultural University (India) has released two hot peppers named Ujwala and Anugraha which show high level field resistance to this pathogen. The variety Anugraha was developed through backcross breeding between a high yielding but highly susceptible variety Pusa Jwala with the highly resistant Ujwala, using Pusa Jwala as a recurrent parent. Thus, Pusa Jwala and Anugraha are near isogenic lines (NILs) differing for the resistance to bacterial wilt only and the resistance is governed by a homozygous recessive (rr) gene action. The F1s of Anugraha × Pusa Jwala were selfed to generate the segregating F2 population. The F2 population has been field screened, 10 highly susceptible and 10 most resistant plants were identified and DNA from these plants were bulked separately. Bulked segregant analysis with AFLP primer combination EcoACT + MseCAC was done using the DNA from donor parent Ujwala, susceptible parent Pusa Jwala, resistant parent Anugraha, bulked susceptible F2 and bulked resistant F2 plants. On resolution using capillary electrophoresis system in genetic analyzer, the AFLP products have yielded three polymorphic bands (103, 117, and 161 bp) which were linked with the resistant recessive allele and three polymorphic bands (183, 296, 319 bp) linked with the dominant susceptible allele of the bacterial wilt resistance gene. The results were confirmed through co-segregation analysis in most resistant and susceptible plants of F2 segregating population. |
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Keywords: | AFLP Bacterial wilt Chilli Bulked segregant analysis Marker assisted selection AFLP"} {"#name":"keyword" "$":{"id":"kwrd0050"} "$$":[{"#name":"text" "_":"amplified fragment length polymorphism BSA"} {"#name":"keyword" "$":{"id":"kwrd0060"} "$$":[{"#name":"text" "_":"bulked segregant analysis BW"} {"#name":"keyword" "$":{"id":"kwrd0070"} "$$":[{"#name":"text" "_":"bacterial wilt NIL"} {"#name":"keyword" "$":{"id":"kwrd0080"} "$$":[{"#name":"text" "_":"near isogenic lines PAGE"} {"#name":"keyword" "$":{"id":"kwrd0090"} "$$":[{"#name":"text" "_":"polyacrylamide gel electrophoresis |
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