| 香蕉愈伤组织原生质体分离研究 |
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| 引用本文： | 李敬阳,张建斌,徐碧玉,金志强. 香蕉愈伤组织原生质体分离研究[J]. 分子植物育种, 2008, 6(4) |
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| 作者姓名： | 李敬阳 张建斌 徐碧玉 金志强 |
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| 作者单位： | 中国热带农业科学院热带生物技术研究所,海口,571101;中国热带农业科学院热带生物技术研究所,海口,571101;中国热带农业科学院热带生物技术研究所,海口,571101;中国热带农业科学院热带生物技术研究所,海口,571101 |
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| 摘 要： | 本实验以巴西香蕉和皇帝蕉胚性愈伤组织为材料,对影响其原生质体产率和活力的因素进行了研究.结果表明:对诱导巴西蕉及皇帝蕉胚性愈伤进行原生质体分离,分别用CPW 13%甘露醇 1.6%纤维素酶 0.9%果胶酶酶解液处理巴西蕉愈伤组织12 h,用CPW 11%甘露醇 1.8%纤维素酶 1.0%果胶酶酶解液处理皇帝蕉愈伤组织12 h.巴西蕉产率最高达2.56×107个/mL,活力达61.5%;皇帝蕉产率达2.64×107个/mL,活力达63.4%..
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| 关 键 词： | 香蕉 愈伤组织 原生质体 分离 |
Study on Embryogenic Callus Protoplast Isolation of Banana |
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| Li Jingyang,Zhang Jianbin,Xu Biyu,Jin Zhiqiang. Study on Embryogenic Callus Protoplast Isolation of Banana[J]. Molecular Plant Breeding, 2008, 6(4) |
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| Authors: | Li Jingyang Zhang Jianbin Xu Biyu Jin Zhiqiang |
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| Affiliation: | Li Jingyang Zhang Jianbin Xu Biyu Jin Zhiqiang Institute of Tropical Bioscience , Biotechnology,Chinese Academy of Tropical Agricultural Sciences,Haikou,571101 |
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| Abstract: | Protoplasts of Musa AAA group cv. Brazilian and Musa paradisiacal AA were isolated from embryogenic calli. The different factors affecting yield and viability of the protoplasts were investigated. The results showed that the protoplasts with higher yield and viability were obtained by treating the Musa AAA group cv. Brazilian and Musa paradisiacal AA embryogenic calli with a mixture of celluase onozuka and macerozyme onozuka. The Musa AAA group cv. Brazilian callus with CPW+13% mannitol+1.6% celluase onozuk... |
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| Keywords: | Banana Callus Protoplast Isolation |
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