运输应激猪骨骼肌中热应激蛋白HSP70和HSP90家族的表达

利用Western blot技术，检测长途运输试验猪骨骼肌（背最长肌和臀长肌）中分属于HSP70家族和HSP90家族的4种应激蛋白（HSP70，HSP72，HSP86和HSP90）的表达，所有运输应激猪和对照猪肌肉组织 中均检测到了上述4种HSP，对照猪组织中HSP的表达说明HSP除了在受应激 的细胞内行使生理功能外，还具有独立于应激刺激应答以外的其它作用，6h长途运输后，HSP的表达量明显不同，HSP70和HSP72在肌肉组织中的表达虽然有一定的增加，但统计学分析差异不显（P＞0．05），而HSP86和HSP90在骨髓肌中的表达明显下降，尤以HSP90下降最显（P＜0．01），提示HSP90家族成员与肉品品质相关，可能作为判应激损伤的的指征。

Expression of HSP70 family and HSP90 family in skeletal muscles of transport stressed pigs

To demonstrate the expression of heat shock proteins(HSP)in skeletal muscles and to estimate the relationship between expression of HSP and pork quality, using Western blot four kinds of HSP which belongs to HSP 70 family and HSP 90 family respectively in porcine skeletal muscle tissues were determined after a long distance transport.All four HSP,namely HSP 70 ,HSP 72 ,HSP 86 and HSP 90 ,were regularly detected in M.longissimus dorsi and M.gluteus medius of both the transported and the control group.The expression of HSP in control pigs showed that HSPs have some independent physiological from stress response undetermined functions.after 6 h transport,the induction of the HSP 70 and HSP 72 in skeletal muscle tissues was slightly elevated( P >0 05) However,expression of the HSP 86 and the HSP 90 ,especially HSP 90 ,markedly decreased( P <0 01)in skeletal muscles.The results are implied that the reduction tendency of HSP 90 family is relative to pork quality and may serve as a potential marker for stress response.