柑橘黄龙病nested-PCR检测技术在柑橘苗木生产中的应用

柑橘黄龙病(huanglongbing, HLB)是柑橘生产上的一种毁灭性病害。本研究以柑橘叶脉为材料，采用nested-PCR技术对广西部分柑橘苗圃苗木进行了黄龙病检测。结果表明，nested-PCR技术不仅可以检测已经表现症状的苗木样品，还可以检测出带病但不显症的苗木样品。2007年共检测柑橘苗木样品1 950个，2008年共检测样品1 480个，黄龙病苗木检出率分别为10.31％和6.22％。本研究对及早控制带病苗木的传播，繁育柑橘无病毒苗木具有重要的意义。

Nested-PCR detection of the citrus huanglongbing disease

The citrus huanglongbing (HLB) is a destructive disease of citrus. Some citrus nursery trees in Guangxi were detected with nervation materials by nested-PCR. Both symptomatic leaves and asymptomatic leaves with HLB could produce positive nested-PCR products. One thousand nine hundred and fifty nursery trees and 1 480 nursery trees were detected during 2007-2008. The incidence of HLB disease was 10.31％ and 6.22％, respectively. A fast and reliable method for HLB pathogen detection has been established, which might play an important role in preventing HLB infected nursery seedlings from spreading.