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报春石斛再生体系的建立
引用本文:张莹,王雁,李振坚.报春石斛再生体系的建立[J].浙江农林大学学报,2009,26(4):603-606.
作者姓名:张莹  王雁  李振坚
作者单位:1.中国林业科学研究院 林业研究所,北京 100091;2.中国林业科学研究院 亚热带林业研究所,浙江 富阳 311400
摘    要:以茎段为外植体,建立了报春石斛Dendrobium primulinum的离体培养再生体系。结果表明:愈合组织诱导以培养基MS(Murashige and Skoog) + 5.0 mg·L-1 6-苄氨基嘌呤(6-BA) + 1.5 mg·L-12,4-D为最优,诱导率达33.33%;分化和增殖培养基均以MS + 1.0 mg·L-16-BA + 0.1 mg·L-1萘乙酸(NAA) +体积分数10%椰子水为佳,分化率最高可达83.20%,增殖倍数达3.82,外源物质椰子水可促进不定芽的形成及增殖。形成的无根苗转移到1/2MS + 0.5 mg·L-16-BA + 1.0 mg·L-1 NAA + 0.5 g·L-1活性炭培养基上诱导生根发育形成完整植株。图1表4参12

关 键 词:植物学    报春石斛    茎段    再生体系

Regeneration system for Dendrobium primulinum
ZHANG Ying,WANG Yan,LI Zhen-jian.Regeneration system for Dendrobium primulinum[J].Journal of Zhejiang A&F University,2009,26(4):603-606.
Authors:ZHANG Ying  WANG Yan  LI Zhen-jian
Institution:1.Research Institute of Forestry,The Chinese Academy of Forestry,Beijing 100091,China;2.Research Institute of Subtropical Forestry,The Chinese Academy of Forestry,Fuyang 311400,Zhejiang,China
Abstract:An in vitro plant regeneration system of Dendrobium primulinum was established using stem sections as explants. Results showed that the optimum callus inducing medium was Murashige and Skoog (MS) + 5.0 mg·L-1 6-BA + 1.5 mg·L-1 2,4-D with an induction rate of 33.3%. Adventitious buds were effectively generated and proliferated on MS + 1.0 mg·L-16-BA + 0.1 mg·L-1 NAA + coconut water 10% with a differentiation rate of 83.2% and proliferation of 3.82 times. Exogenous coconut water promoted adventitious bud generation and proliferation. The best rooting medium was 1/2MS + 0.5 mg·L-1 6-BA +1.0 mg·L-1NAA + 0.5 g·L-1 activated carbon. [Ch,1 fig. 4 tab. 12 ref.]
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