|
|||||
|
|
| 国内外甘蓝型油菜种质SSR标记遗传多样性分析 | |
| 引用本文： | 王凯华 张文英 王会 张雄. 国内外甘蓝型油菜种质SSR标记遗传多样性分析[J]. 中国农学通报, 2011, 27(19): 144-149 |
| 作者姓名： | 王凯华 张文英 王会 张雄 |
| 作者单位： | 长江大学长江中游湿地农业教育部工程研究中心,湖北荆州,434025 |
| 基金项目： | 湖北省教育厅科学技术研究计划优秀中青年人才项目(Q20101318) |
| 摘 要： | 用SSR分子标记对国内外48份甘蓝型油菜品种进行遗传多样性分析，结果表明：筛选出的45对引物共扩增出326个位点，多态位点281个，多态性比率为86.2%；平均每对引物扩增的条带数和多态性条带数分别为7.2和6.2。多态性信息含量（PIC）在0.374~0.856，平均为0.699。遗传相似系数在0.48~0.79之间，参试材料差异较大；以0.51为阈值将48份参试材料划分为冬性、半冬性和春性三大类群，三大类群间相对独立又有一定程度的渗透，说明材料之间存在不同程度的亲缘关系。主成分分析与聚类分析结果一致。说明SSR标记能够较全面地反应种质材料的遗传多样性，能够为种质的保存提供帮助，同时可以用来分析育种材料的遗传多样性，对育种工作有重要的参考意义。 |
| 关 键 词： | 银杏 银杏 正交试验 愈伤组织 银杏叶汁 银杏胚乳汁 |
| 收稿时间： | 2011-04-26 |
| 修稿时间： | 2011-06-24 |
Genetic Diversity Analysis of Domestic and Foreign Rapeseed (Brassica napus L.) by SSR Markers |
|
| Wang Kaihua,Zhang Wenying,Wang Hui,Zhang Xiong. Genetic Diversity Analysis of Domestic and Foreign Rapeseed (Brassica napus L.) by SSR Markers[J]. Chinese Agricultural Science Bulletin, 2011, 27(19): 144-149 | |
| Authors: | Wang Kaihua Zhang Wenying Wang Hui Zhang Xiong |
| Affiliation: | Wang Kaihua,Zhang Wenying,Wang Hui,Zhang Xiong ( Engineering Research Center of Wetland Agriculture in the Central Yangtze of Ministry of Education,Yangtze University,Jingzhou Hubei 434025) |
| Abstract: | The genetic diversity of 48 rapeseed (Brassica napus L.), which were from domestic and foreign, was analyzed by SSR markers. The results showed that 45 pairs of primers performed 326 allele loci, and the percentage of polymorphism locus was 86.2% with 281 loci was detected. The average number of allele and polymorphism loci per primer was 7.2 and 6.2, respectively. The range of PIC per primer was 0.374-0.856, with the average of 0.699. Difference among the accessions was large with the genetic similarity ra... |
| Keywords: | rapeseed (Brassica napus L.) SSR marker genetic diversity |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |
| 点击此处可从《中国农学通报》浏览原始摘要信息 | |
| 点击此处可从《中国农学通报》下载全文 | |