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| 金银花、山银花绿原酸类提取物体外抗NDV作用研究 | |
| 引用本文： | 王林青,张红英,崔保安,李文增,李坤陶.金银花、山银花绿原酸类提取物体外抗NDV作用研究[J].中国农学通报,2011,27(19):277-282. |
| 作者姓名： | 王林青 张红英 崔保安 李文增 李坤陶 |
| 作者单位： | 1. 郑州师范学院生命科学系,郑州450044 ;河南农业大学牧医工程学院,郑州450002 2. 河南省动物性食品安全重点实验室,郑州450002;河南农业大学牧医工程学院,郑州450002 3. 郑州师范学院生命科学系,郑州,450044 |
| 基金项目： | 郑州市创新型科技人才队伍建设工程(10CXTD148); 郑州师范学院青年课题基金资助项目(2010QNZZ014) |
| 摘 要： | 为研究比较价格差异较大的金银花和山银花的体外抗病毒作用，分别提取金银花和山银花绿原酸类活性成分，利用Vero细胞体外培养系统，通过观察细胞病变效应（CPE），并用MTT法检测细胞活性来评价和比较金银花和山银花绿原酸类提取物体外抑制新城疫病毒（NDV）对细胞的感染作用。结果表明：在安全浓度范围内，金银花和山银花绿原酸类提取物均具有显著的抗病毒作用，体外对NDV分别具有明显的阻断作用、抑制作用和中和作用，且两者之间的抗病毒效果差异不显著。本试验为价格相对便宜的山银花在一定领域部分代替金银花提供了实验依据。 |
| 关 键 词： | 水稻 耐冷 指标 QTL定位 水稻 耐冷 指标 QTL定位 |
| 收稿时间： | 3/4/2011 12:00:00 AM |
| 修稿时间： | 2011/3/31 0:00:00 |
The Study on Antiviral Effect of Chlorogenic Acids from Flos Lonicerae japonicae and Flos lonicerae on NDV in Vitro |
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| Wang Linqing,Zhang Hongying,Cui Bao'an,Li Wenzeng,Li Kuntao.The Study on Antiviral Effect of Chlorogenic Acids from Flos Lonicerae japonicae and Flos lonicerae on NDV in Vitro[J].Chinese Agricultural Science Bulletin,2011,27(19):277-282. | |
| Authors: | Wang Linqing Zhang Hongying Cui Bao'an Li Wenzeng Li Kuntao |
| Institution: | 1 Department of Life Science, Zhengzhou Normal University , Zhengzhou 450044; 2 Key Laboratory of Henan Province Animal Food Safety , Zhengzhou 450002; 3 College of Animal Husbandry and Veterinary Medicine, Henan Agricultural University , Zhengzhou 450002) |
| Abstract: | The aim was to study the antiviral effects between Flos Lonicerae japonicae and Flos lonicerae which were very different in price. Chlorogenic acids were extracted from them. The antiviral effects of the chlorogenic acids extracts on the infection of Newcastle disease virus (NDV) to cells were investigated by observing cytopathic effect (CPE) in Vero cell, and the cell survival rate by MTT method was detected. The antivirus effects of Flos Lonicerae japonicae and Flos Lonicerae were compareed. The results s... |
| Keywords: | Flos Lonicerae japonicae Flos lonicerae NDV chlorogenic acids Vero |
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