| 人参中ACE抑制蛋白的提取 |
| |
| 引用本文： | 宋晶,李海涛,程海涛,张秋菊,张爱华,张连学.人参中ACE抑制蛋白的提取[J].中国农学通报,2011,27(19):133-136. |
| |
| 作者姓名： | 宋晶 李海涛 程海涛 张秋菊 张爱华 张连学 |
| |
| 作者单位： | 吉林农业大学中药材学院,长春,130118 |
| |
| 基金项目： | 国家科技支撑计划“平地栽参关键技术研究”(NO.2007BAI38B01) |
| |
| 摘 要： | 从人参粉中提取纯化人参蛋白，以检测人参蛋白对血管紧张素转换酶（ACE）的抑制活性。将人参粉加水（pH 9.0）搅拌，3000 r/min离心20 min。用连续的色谱柱分离：D101大孔树脂分离色谱，离子交换色谱，凝胶过滤色谱。以体外ACE抑制率为指标确定提取的人参蛋白具有抑制ACE的作用。结果表明：人参蛋白对ACE酶具有很高的抑制效果，通过连续的色谱分离方法可将ACE抑制蛋白分离，得到具有较高抑制率的ACE抑制蛋白，该抑制蛋白的抑制率可达92.5%。
|
| 关 键 词： | 主成分分析 主成分分析 |
| 收稿时间： | 2011/1/17 0:00:00 |
| 修稿时间： | 2011/2/27 0:00:00 |
Extraction of Angiotensin I-converting Enzyme Inhibitory Protein from Ginseng |
| |
| Song Jing,Li Haitao,Cheng Haitao,Zhang Qiuju,Zhang Aihua,Zhang Lianxue.Extraction of Angiotensin I-converting Enzyme Inhibitory Protein from Ginseng[J].Chinese Agricultural Science Bulletin,2011,27(19):133-136. |
| |
| Authors: | Song Jing Li Haitao Cheng Haitao Zhang Qiuju Zhang Aihua Zhang Lianxue |
| |
| Institution: | Song Jing,Li Haitao,Cheng Haitao,Zhang Qiuju,Zhang Aihua,Zhang Lianxue ( College of Tradition Chinese Medicine,Jilin Agricultural University,Changchun 130118) |
| |
| Abstract: | The ginseng protein was extracted from ginseng powder, and the inhibitory activity of angiotensin I-converting enzyme (ACE) was detected. Ginseng powder was prepared by stirring in water (pH 9.0), followed by centrifugation at 3000 r/m for 20 min. An ACE inhibitor was purified by using consecutive chromatographic methods, including: D101 macroporous resin chromatography, ion-exchange chromatography, and gel filtration chromatography. The inhibitory activity of ACE was determined as ACE inhibitory activity i... |
| |
| Keywords: | ginseng angiotensin I-converting enzyme inhibitor inhibitory ratio bioactive peptide |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《中国农学通报》浏览原始摘要信息 |
| 点击此处可从《中国农学通报》下载免费的PDF全文 |
|
