| 环境中胆固醇降解菌的分离与鉴定 |
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| 引用本文： | 艾育芳,潘大仁,周以飞,彭康,李姜平.环境中胆固醇降解菌的分离与鉴定[J].中国农学通报,2011,27(18):139-142. |
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| 作者姓名： | 艾育芳 潘大仁 周以飞 彭康 李姜平 |
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| 作者单位： | 福建农林大学作物遗传育种与综合利用教育部重点实验室,福州,350002 |
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| 基金项目： | 福建省自然科学基金项目“睾丸酮丛毛单胞菌teiR基因在内固降解途径中的作用研究”(2008J0041); 教育部科学技术重点项目“高效降解类固醇的工程菌菌株的改造与筛选”(No.207056) |
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| 摘 要： | 本研究利用富集培养法从水塘底的污泥中分离出12株能以胆固醇为唯一碳源生长的菌株，并初步筛选出4株降解能力较强的菌株，这4个菌株在30℃，200 r/min的培养条件下，96 h内对30 μg/mL的胆固醇的降解率分别为51.25%、36.65%、55.62%和29.59%。且它们适合生长的pH范围广，均在第12 h后进入生长的稳定期。其中2个菌株（L4、L5）经形态特征以及16SrDNA序列分析，初步鉴定为肺炎克氏杆菌（Klebsiella pneumoniae）。
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| 关 键 词： | 社区增权 社区增权 |
| 收稿时间： | 4/2/2011 12:00:00 AM |
| 修稿时间： | 2011/4/25 0:00:00 |
Isolation and Identification of Cholesterol-Degrading Strains in the Environment |
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| ZHOU Yi-Fei.Isolation and Identification of Cholesterol-Degrading Strains in the Environment[J].Chinese Agricultural Science Bulletin,2011,27(18):139-142. |
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| Authors: | ZHOU Yi-Fei |
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| Institution: | Ai Yufang,Pan Daren,Zhou Yifei,Peng Kang,Li Jianqping (Key Laboratory of Ministry of Education for Genetics,Breeding and Multiple Utilization of Crops,Fujian Agriculture and Forestry University,Fuzhou 350002) |
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| Abstract: | In this paper,12 strains that utilized cholesterol as sole carbon and energy sources were isolated from the sludge by the enrichment culture method.Four strains were selected for degradation of cholestero1.In the culture conditions of 30℃ and 200 r/min,the degradation rate to cholesterol of 4 strains were 51.25%,36.65%,55.62% and 29.59% respectively,with 30 μg/mL concentrations within 96 hours.The suitable pH values for their growth were wide,and the strains went into the stationary phase at the twelfth hour.L4 and L5 strains were identified to be Klebsiella pneumoniae by morphological characteristics and the similarity analysis of the 16S rDNA sequence. |
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| Keywords: | bioremediation degrading bacteria cholesterol isolation 16S rDNA |
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