| 实验猴粪便样品中沙门氏菌LAMP检测方法的建立及应用简 |
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| 引用本文： | 邱索平,林志雄,游勇来,等. 实验猴粪便样品中沙门氏菌LAMP检测方法的建立及应用简[J]. 中国畜牧兽医, 2014, 41(1): 51-56 |
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| 作者姓名： | 邱索平 林志雄 游勇来 等 |
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| 摘 要： |
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| 关 键 词： | 沙门氏菌 环介导等温扩增 粪便样品 实验猴 |
Development and Application of Loop-mediated Isothermal Amplification Assay for Salmonella Detection in Fecal Samples of Experimental Monkeys |
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| QIU Suo-ping,LIN Zhi-xiong,YOU Yong-lai,MA Bao-hua,ZHAO Qiong. Development and Application of Loop-mediated Isothermal Amplification Assay for Salmonella Detection in Fecal Samples of Experimental Monkeys[J]. China Animal Husbandry & Veterinary Medicine, 2014, 41(1): 51-56 |
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| Authors: | QIU Suo-ping LIN Zhi-xiong YOU Yong-lai MA Bao-hua ZHAO Qiong |
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| Abstract: | A loop-mediated isothermal amplification (LAMP) assay was developed for detection of Salmonlla in fecal samples of experimental monkeys. According to the specific sequences (fimY) of Salmonlla in GenBank, one set of primers was selected and the reaction condition was optimized. The results showed that the detection limit of LAMP method was 1.35×101 and 1.35×103 CFU/mL in Salmonella pure culture and clinical samples,respectively,which was the same as routine PCR. The amplification could complete in one hour, and the result could be distinguished by naked eyes. There was non-specific amplification of other pathogens. These results suggested that this LAMP assay was a simple and specific method for rapid detection of Salmonlla in fecal samples. |
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| Keywords: | Salmomella LAMP fecal samples experimental monkeys |
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