| 育亨宾生物总碱的分离与纯化 |
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| 引用本文： | 王锦雨,张耸,崔连雷.育亨宾生物总碱的分离与纯化[J].北京农业,2011(27):7-10. |
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| 作者姓名： | 王锦雨 张耸 崔连雷 |
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| 作者单位： | 山东农业大学生命科学学院 |
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| 摘 要： | 本实验旨在将低含量的育亨宾生物碱纯化、富集,并分离其多种成分。选用8.00%育亨宾盐的粗品,先用酸溶碱沉和有机物萃取的提取方法得到较高含量的育亨宾生物碱,即纯化的育亨宾生物碱。然后用紫外分光光度法粗略的测定纯化的育亨宾生物碱的含量,再通过超高效液相色谱法精确的测定纯品的育亨宾生物碱含量。其分离可通过配置合适的洗脱液,过碱性氧化铝的柱子实现。最终获得纯度较高的育亨宾生物碱,并收集到其在柱层析过程分离开的各条带,即育亨宾生物碱的各种同分异构体,将其各种成分成功的分离开。经过碱性氧化铝的柱层析,将纯化的育亨宾生物碱分离为两条色谱带。
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| 关 键 词： | 生物碱 育亨宾 纯化 分离 |
Yohimbine Alkaloids Separation and Purification |
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| Wang Jinyu Zhang Song Cui Lianlei.Yohimbine Alkaloids Separation and Purification[J].Beijing Agriculture,2011(27):7-10. |
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| Authors: | Wang Jinyu Zhang Song Cui Lianlei |
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| Institution: | Wang Jinyu Zhang Song Cui Lianlei |
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| Abstract: | This experiment is designed to purify and separate the various components of yohimbine alkaloid.Using the 8% yohimbine salt crude,firstly purify it by acid-solute-alkali-sink and organic extraction method to get a higher extraction of yohimbine alkaloid.Then determine the concentration of yohimbine by UV spectrophotometry and high efficient liquid chromatography.Lastly,use the alumina column chromatography to separate the different isomers of yohimbine alkaloid into two bands. |
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| Keywords: | alkaloids yohimbine purification separation |
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