| 思茅松成熟胚的胚性愈伤组织诱导与增殖1） |
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| 引用本文： | 耿菲菲,肖丰坤,吴涛,陈芳,杨宇明,王娟.思茅松成熟胚的胚性愈伤组织诱导与增殖1）[J].东北林业大学学报,2015(5):59-63. |
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| 作者姓名： | 耿菲菲 肖丰坤 吴涛 陈芳 杨宇明 王娟 |
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| 作者单位： | 1. 西南林业大学，昆明，650224;2. 云南省林业科学院 |
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| 基金项目： | 1)国家林业公益性行业科研专项(201304105)；云南省应用基础研究计划重点项目(2013FA054)；云南省中青年学术技术带头人后备人才培养项目(2010CI016)。 |
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| 摘 要： | 以思茅松（Pinus kesiya var．langbianensis）成熟合子胚为外植体，1／2MSG培养基为基本成分，采用正交试验设计研究2，4－D、6－BA和TRIA 3种植物生长调节剂对其胚性愈伤组织诱导的影响并获得最优诱导培养基，通过改变生长调节剂种类和降低其质量浓度的方式研究胚性愈伤组织增殖效果，筛选适宜的增殖培养基。结果表明：1／2MSG＋2，4－D 5．000 mg· L－1＋6－BA 4．000 mg· L－1＋TRIA 0．005 mg· L－1为最佳诱导培养基；2，4－D对思茅松成熟胚诱导胚性愈伤组织的影响最大，其次是TRIA，6－BA的影响最小；最佳的增殖培养基为P6＋NAA 2．0 mg· L－1＋6－BA 0．6 mg· L－1。
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| 关 键 词： | 思茅松 成熟胚 体细胞胚胎发生 胚性愈伤 |
Ind uction and Proliferation of Embryonic Calluses from Mature Zygotic Embryos of Pinus kesiya var.langbianen-sis |
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| Geng Feifei,Xiao Fengkun,Wu Tao,Chen Fang,Yang Yuming,Wang Juan.Ind uction and Proliferation of Embryonic Calluses from Mature Zygotic Embryos of Pinus kesiya var.langbianen-sis[J].Journal of Northeast Forestry University,2015(5):59-63. |
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| Authors: | Geng Feifei Xiao Fengkun Wu Tao Chen Fang Yang Yuming Wang Juan |
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| Abstract: | The embryonic calluses were initiated and proliferated from mature zygotic embryos of Pinus kesiya var.langbianensis on 1/2MSG basical medium.The effects of three types of plant growth regulators (PGR), 2,4-D, BA and TRIA, on callu-ses induction and proliferation were conducted by orthogonal design experiments .The best medium for the embryonic callu-ses induction was 1/2MSG+2,4-D 5 mg· L-1+BA 4 mg· L-1+TRIA 0.005 mg· L-1 .Among three PGRs on embryogenic callus induction, 2,4-D was the most significant one, followed by TRIA, and 6-BA was minimum.The optimum for prolif-eration was P 6+NAA 2 mg· L-1+6-BA 0.6 mg· L-1 . |
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| Keywords: | Pinus kesiya var langbianensis Mature embryo Somatic embryogenesis Embryonic callus |
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