大戟、芫花反甘草在小鼠急性肝损伤条件下的毒性研究

以健康小鼠腹腔注射法测定单味大戟、芫花与甘草及其不同比例的半数致死量(LD_(50))。用4％CCl_4皮下注射法使小鼠发生急性肝损伤,再以上述中药LD_(50)对这些小鼠进行毒性实验。动物实验结果表明,大戟、芫花分别与甘草以不同比例配伍给予健康实验动物时,其毒性增强。在肝损伤小鼠,其情况也是一样,但当大戟、芫花与甘草等量配伍时,其毒性降低。若口服给药,动物机体无毒性反应。显然,这些中药的配伍毒性和用药途径有关。

Toxicity Studies on the Three Chinese Medicinal Herbs in Mice Suffered from Acute Liver Injury

The median lethal doses(LD_(50)) of single Knoxia valerianoides Thorel. et Pitard,Daphne genkwa Sieb. et Zucc,Glycyrrhiza uralensis Fisch and their various proportions were determined with the method of peritoneal injection in healthy mice. The toxicity experiments with LD_(50) of the above Chinese medicinal herbs were carried out on mice suffered from acute liver injury caused by hypodermatic injection of 4% CCl_4, The results showed that when Knoxia valerianoides or Daphne genkwa was matched with Glycyrrhiza uralensis respectively and given to the experimental animals at different dosages, a marked toxicity reaction was observed. There was a similar circumstance in the mice suffered from liver injury, The toxicity reaction decreased when Knoxia valerianoides or Dephne genkwa cooperated with Glycyrrhiza uralensis at equal proportion. While the herbs were given orally, no toxic effect was observed, It is suggested that the toxicity of the Chinese medicinal herbs used cooperately may be in relation to their administration routes.