| 亲和层析纯化猪胰蛋白酶的研究 |
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| 引用本文： | 孟国良.亲和层析纯化猪胰蛋白酶的研究[J].郑州牧业工程高等专科学校学报,1996(3). |
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| 作者姓名： | 孟国良 |
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| 作者单位： | 郑州牧专畜牧工程系 |
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| 摘 要： | 采用猪胰蛋白酶的天然抑制剂-鸡卵类粘蛋白(CHOM)作为配基合成亲和吸附剂,从猪胰脏的粗提液中,通过亲和层析直接获得高纯度的胰蛋白酶.此法具有操作简便、产品纯度、比活力和获得率高等优点.
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| 关 键 词： | 配基 胰蛋白酶 鸡卵类粘蛋白 亲和层析 比活力 |
STUDIES ON PURIFICATION OF TRYPSIN FROM PORCINE PANCREAS BY AFFINITY GEL CHROMATOGRAPHY |
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| Meng Guoliang.STUDIES ON PURIFICATION OF TRYPSIN FROM PORCINE PANCREAS BY AFFINITY GEL CHROMATOGRAPHY[J].Journal of Zhengzhou College of Animal Husbandry Engineering,1996(3). |
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| Authors: | Meng Guoliang |
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| Institution: | Zhengzhou College of Animal Husbandry |
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| Abstract: | A method is presented for the purification of trypsin from crude extracts of porcine pancreas by an affinity chromatography on the CHOM - Sepharose 4B, CHOM is the natural inhibitor of porcine trypsin. The results have showed that it is a simple and time - saving method for the purification of trypsin with high purity, activity and yield. |
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| Keywords: | Ligand Trypsin CHOM Affinity gel chromatography Comparative activity |
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