| 密闭气袋在牛卵母细胞体外成熟培养中的应用 |
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| 引用本文： | 赵贵民,吴苏君,刘文强,井勇,杨文琳,高志敏,雷安民.密闭气袋在牛卵母细胞体外成熟培养中的应用[J].中国兽医学报,2011,31(9). |
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| 作者姓名： | 赵贵民 吴苏君 刘文强 井勇 杨文琳 高志敏 雷安民 |
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| 作者单位： | 西北农林科技大学动物医学院陕西省干细胞工程技术研究中心陕西省农业分子生物学重点实验室,陕西杨凌,712100 |
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| 基金项目： | 国家“863”计划资助项目(2008AA101010) |
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| 摘 要： | 通过简易避光密闭气袋在人体呼出的气相环境下（CO2浓度约5%,O2浓度约15%）,利用生化培养箱进行牛卵母细胞的体外成熟培养。结果显示,牛卵母细胞在密闭气袋中培养与对照组（正常培养）相比成熟率（74.79%比71.69%,P〉0.05）、孤雌激活后卵裂率（83.07%比81.39%,P〉0.05）和囊胚发育率上（26.75%比22.87%,P〉0.05）均无显著差异,但是密闭气袋培养各项数据均略高于对照组。本试验验证了该技术的方便性和有效性,为此技术在生产实践中的推广应用提供依据。
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| 关 键 词： | 密闭气袋 牛 卵母细胞 体外成熟 低氧 |
Use of airtight spirit bag for bovine oocytes in vitro maturation |
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| ZHAO Gui-min,WU Su-jun,LIU Wen-qiang,JING Yong,YANG Wen-lin,GAO Zhi-min,LEI An-min.Use of airtight spirit bag for bovine oocytes in vitro maturation[J].Chinese Journal of Veterinary Science,2011,31(9). |
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| Authors: | ZHAO Gui-min WU Su-jun LIU Wen-qiang JING Yong YANG Wen-lin GAO Zhi-min LEI An-min |
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| Institution: | ZHAO Gui-min,WU Su-jun,LIU Wen-qiang,JING Yong,YANG Wen-lin,GAO Zhi-min,LEI An-min(Shaanxi Stem Cell Engineering and Technology Research Center,Shaanxi Key Laboratory of Molecular Biology for Agriculture,College of Veterinary Medicine Northwest A & F University,Yangling,Shaanxi 712100,China) |
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| Abstract: | In this study,human breath gas environment that appropriate concentration of CO2(5%) and the lower oxygen concentration(15%) was used for bovine oocytes in vitro maturation by airtight spirit bag and biochemical incubator.The results showed there were no significantly difference between bovine oocytes cultured in airtight spirit bag and the control(the normal cultured oocyctes) in the maturation rate(74.79% vs.71.69%,P>0.05),cleavage rate(83.07% vs.81.39%,P>0.05) and blastocyst rate(26.75% vs.22.87%,P>0.05)... |
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| Keywords: | airtight spirit bag bovine oocytes in vitro maturation hypoxia |
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