| 杀线虫苏云金芽胞杆菌菌株YC-10的分离鉴定及活性测定 |
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| 引用本文： | 成飞雪,王忠勇,刘勇,张德咏,程菊娥.杀线虫苏云金芽胞杆菌菌株YC-10的分离鉴定及活性测定[J].中国生物防治,2011,27(4):540-546. |
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| 作者姓名： | 成飞雪 王忠勇 刘勇 张德咏 程菊娥 |
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| 作者单位： | 湖南省植物保护研究所,长沙,410125 |
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| 基金项目： | 公益性行业(农业)科研专项(nyhyz07050); 国家自然科学基金(3080717) |
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| 摘 要： | 自烟草中分离获得对南方根结线虫Meloidogyneincognita具有较高杀线活性的拮抗细菌菌株YC-10。研究发现该菌株有伴胞晶体产生，菌体、芽胞形态及生理生化特性与苏云金芽胞杆菌库斯塔克亚种B．thuringiensissubsp．kurstaki相似，16srDNA序列分析表明其与苏云金芽胞杆菌相似性最高，初步确定该菌株为苏云金芽胞杆菌。菌株发酵液及伴胞晶体蛋白对南方根结线虫J2有很强的杀线活性，发酵液处理南方根结线虫，24h线虫死亡率为100％；伴胞晶体蛋白处理南方根结线虫，24h的LC50为5．86μg．mL^-1。研究结果显示该菌株在线虫生物防治上有较大的应用潜力。
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| 关 键 词： | 苏云金芽胞杆菌 鉴定 杀线活性 生物防治 |
Identification and Nematicidal Activities of Bacillus thuringiensis Strain YC-10 |
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| CHENG Fei-xue,WANG Zhong-yong,LIU Yong,ZHANG De-yong,CHENG Ju-e.Identification and Nematicidal Activities of Bacillus thuringiensis Strain YC-10[J].Chinese Journal of Biological Control,2011,27(4):540-546. |
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| Authors: | CHENG Fei-xue WANG Zhong-yong LIU Yong ZHANG De-yong CHENG Ju-e |
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| Institution: | CHENG Fei-xue,WANG Zhong-yong,LIU Yong,ZHANG De-yong,CHENG Ju-e(Hunan Plant Protection Institute,Changsha 410125,China) |
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| Abstract: | The bacterium strain YC-10 isolated from a tobacco sample demonstrated high toxicity against Meloidogyne incognita.The strain could produce protein crystals.The morphology of the strain and spores and physiological-biochemical features were similar to those of B.thuringiensis subsp.kurstaki.In combination with 16S rDNA analysis,the strain was identified as B.thuringiensis.Bioassay results showed that the culture filtrate and the parasporal crystal proteins of the strain were highly toxic to M.javanica J2.Th... |
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| Keywords: | Bacillus thuringiensis identification nematicidal activity biocontrol |
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