罗田苍术增殖培养基的优化

目的]筛选罗田苍术Atractylodes lancea (Thunb.) DC.Subsp.Luotianensis Hu et Feng in ed.]增殖培养的最佳培养基,为罗田苍术种苗工厂化生产提供技术指导。方法]以罗田苍术的嫩枝为外植体,筛选出最适宜的基本培养基;再利用L9(34)正交试验优化6-BA、KT、NAA和IBA的配比。结果]N68为罗田苍术最适宜的基本培养基,各植物生长调节剂的最佳浓度为1.0 mg/L6-BA+1.0 mg/LKT+0.5 mg/LNAA+0.2 mg/LIBA。结论]该试验筛选出了罗田苍术增殖培养的最佳培养配方,为罗田苍术种苗工厂化生产提供了技术指导。

Optimization of Multiplication Culture Medium of Atractylodes lancea (Thunb.)DC.Subsp.Luotianensis Hu et Feng in ed.

Objective]To obtain the optimum medium for the multiplication culture of Atractylodes lancea(Thunb.)DC.Subsp.Luotianensis Hu et Feng in ed,so as to provide technical support for the industrialized production of Atractylode s lancea(Thunb.)DC.Subsp.Luotianensis Hu et Feng in ed.seedlings.Method]By employing tender twigs of Atractylodes lancea(Thunb.)DC.Subsp.Luotianensis Hu et Feng in ed.as explants,the optimal minimum medium was screened out,and the proportion of 6-BA,KT,NAA and IBA was optimized by L9(34) orthogonal test.Result]The optimal minimal medium was N68,and the optimum proportion of each plant growth regulators was 1.0 mg/L 6-BA+1.0 mg/L KT+0.5 mg/L NAA+0.2 mg/L IBA.Conclusion]The optimum medium for the multiplication culture of Atractylodes lancea(Thunb.)DC.Subsp.Luotianensis Hu et Feng in ed.is obtained in this study,which provides technical support for the industrialized production of Atractylodes lancea(Thunb.)DC.Subsp.Luotianensis Hu et Feng in ed.seedlings.