灌喂石榴汁对大鼠体重及粪便和盲肠中鞣花酸、尿石素A含量的影响

目的通过给SD大鼠灌喂石榴汁,研究其体重增加和粪便及盲肠内容中鞣花酸及尿石素A含量,为深层次研究鞣花酸及尿石素A的生物学作用提供数据支持。方法选择24只平均体重为（169.08±7.88）g的雄性SD大鼠,随机分为3组,每组8只,分别为对照组、试验Ⅰ组和试验Ⅱ组。在相同日粮基础上,试验Ⅰ组大鼠每天每只灌喂1.5 mL石榴汁,试验Ⅱ组大鼠每天每只灌喂3.0 mL石榴汁,并获取大鼠体重数据及采集粪便及盲肠内容物样品。结果①试验Ⅱ组大鼠日增重显著（P<0.05）高于对照组,比对照组高36.32%;②灌喂石榴汁显著（P<0.05）增加大鼠粪便鞣花酸和尿石素A的含量;③灌喂石榴汁后,试验Ⅰ组和试验Ⅱ组大鼠盲肠中鞣花酸和尿石素A的含量均极显著（P<0.01）高于对照组。结论给大鼠灌喂石榴汁可以显著提高大鼠体重,显著或极显著提高大鼠粪便和盲肠内容中鞣花酸和尿石素A的含量,且灌喂3 mL/（只·d）效果最佳。

Effects of Pomegranate Juice on Body Weight and Content of Ellagic Acid and Urolithin A in Feces and Cecum Contents in Rats

［Objective］ To determine whether feeding pomegranate juice would have effect on body weight and content of ellagic acid and urolithin A in feces and cecum contents in SD rats, and to provide data support for the in-depth study on biological effects of ellagic acid and urolithin A. ［Method］ A total of 24 male SD rats with an average body weight of （169.08±7.88） g were randomly divided into 3 groups （control group, test groupⅠand test groupⅡ） with 8 rats in each group. All of the excremental rats fed the same basal diet. The rats in test groupⅠand Ⅱ were fed with 1.5 mL and 3.0 mL of pomegranate juice per day, respectively. The rats were weighted and their feces and cecum contents were sampled. ［Result］ The daily weight gain of the rats in test group Ⅱ was increased by 36.32% （P<0.05） compared to the control group. Feeding pomegranate juice significantly （P<0.05） increased the content of ellagic acid and urolithin A in feces of rats, and extremely significantly （P<0.01） increased the content of ellagic acid and urolithin A in cecum contents of rats. ［Conclusion］ Feeding pomegranate juice for rats has significant or extremely significant effects on increasing the body weight and content of ellagic acid and urolithin A in feces and cecum contents, and the best effect was observed in the feeding volume of 3 mL per day per rat.