2株致犊牛腹泻大肠杆菌的分离鉴定和药敏试验

赣西某肉牛养殖场有犊牛出现消瘦、食欲下降和排腥臭稀便等症状,为确定病原采集了腹泻犊牛的新鲜粪便,采用Bio-X犊牛消化道五联原位快速诊断试剂盒进行快速诊断,同时进行病原菌的分离与鉴定;为了确定病原菌对抗菌药的敏感性,选取了磺胺嘧啶钠、青霉素、链霉素、土霉素和氧氟沙星等多种抗菌药对病原菌进行体外抑菌试验。通过临床快速诊断与实验室诊断确定该病是由大肠杆菌引起的,体外抑菌试验结果显示2株分离菌对环丙沙星高度敏感,对青霉素和头孢拉定等抗菌药表现完全耐药。该养殖场依据药敏试验结果合理用药并采取多种防治措施,数日后无新发病例并且发病牛的病情得到较好控制。

Isolation,Identification and Antimicrobial Susceptibility Test of Two Strains of Escherichia coli Causing Calf Diarrhea

Calves in a beef cattle farm in western Jiangxi Province were reported to have clinical symptoms of emaciation, loss of appetite and diarrhea. In order to identify the pathogenic agent associated with this disease, fresh fecal samples of calves with diarrhea were recovered, Bio-x five-line in situ rapid diagnosis kit of calf digestive tract was used for rapid diagnosis, isolation and identification of pathogenic bacteria were carried out, and in vitro susceptibility of the isolates against the commonly used antibiotics in veterinary clinic was tested. Rapid clinical diagnosis and laboratory diagnosis confirmed that the disease was caused by infection of Escherichia coli. The two isolates were highly sensitive to ciprofloxacin and completely resistant to penicillin, cefradine and other antibiotics. The diarrhea of calves in this cattle farm were well controlled using the antibiotic recommended by this study.