转化CaMV基因芸薹属蔬菜植株抗病性鉴定及其遗传分析

 以转化CaMV 弱株系Bari1 基因􀀂 的大白菜、菜薹、紫菜薹和花椰菜植株为试材, 研究了CaMV 弱株系Bari􀀁1 基因􀀂 所提供的遗传工程交叉保护及其遗传规律。结果表明,在转化CaMV 弱株系Bari􀀁1 基因􀀂 的大白菜、菜薹、紫菜薹和花椰菜植株中, 48. 08%对CaMV强株系CABB􀀁BJI 具有较强的抗性, 51. 92%表现为敏感。抗性基因( CaMV Bari􀀁1 基因) 在自交代( S1) 中大多数( 76%) 表现为典型的孟德尔单基因显性遗传, 部分株系的抗性出现了15 1, 1 1, 1 3 和1 95 的非孟德尔遗传现象。

Diagnosis and Genetic Analysis of Resistance to Cauliflower Mosaic Virus in Brassica Crops Which Was Transformed with CaMV Gene VI

The genetically engineered cross􀀁protection which was supplied by weak strain Bari􀀁1 gene VI of cauliflower mosaic virus ( CaMV) , and its genetic regularity were studied with Brassica crops ( Brassica camp estris ssp.rap ifera, B . campestris ssp. pekinensis , B . campestris var . parachinensis , B. campestris var. purpurea, B .juncea , B . oleracea var. botrytic ) which were transformed with CaMV gene VI. The results showed that 48. 08% oftransformed Brassica plants was resistance to CaMV strain CABB􀀁BJI and 51. 92% of them was susceptible to CaMV CABB􀀁BJI. The virus resistant gene ( CaMV Bari􀀁1 gene VI ) in 76% of first selfed progeny presented typicallyMendelian single dominance gene inheritance. The ratio of virus􀀁resistant to virus􀀁susceptible plants in some of first selfed progeny presented Mendelian inheritance, such as, 15 1, 1 1, 1 3 and 1 95.