| Institution: | 1. Jožef Stefan Institute, Jamova cesta 39, SI-1000 Ljubljana, Slovenia;2. Karlsruhe Institute of Technology, Kaiserstraße 12, 76131 Karlsruhe, Germany;3. NUBIKI Nuclear Safety Research Institute, Konkoly-Thege Miklós út 29-33, 1121 Budapest, Hungary;4. Nuclear Regulatory Authority of the Slovak Republic, Bajkalská 27, 82007 Bratislava, Slovakia;5. JSC Atomenergoproekt, 1, st. Podolskykh Kursantov, Moscow, Russian Federation;6. Ricerca sul Sistema Energetico, Via Rubattino 54, 20134 Milano, Italy;7. Lithuania Energy Institute, Breslaujos g.3, 44403 Kaunas, Lithuania;1. Department of Chemical and Process Engineering, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor, Malaysia;2. Fuel Cell Institute, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor, Malaysia;1. Laboratory of Biocatalysis, Kemerovo State University, Krasnaya Street 6, Kemerovo 650043, Russia;2. Department of Bionanotechnology, Kemerovo State University, Krasnaya Street 6, Kemerovo 650043, Russia;3. Research Institute of biotechnology, Kemerovo State University, Krasnaya Street 6, Kemerovo 650043, Russia;4. Department of Higher Mathematics, Kemerovo State University, Krasnaya Street 6, Kemerovo 650043, Russia;5. College of Food and Bioengineering, Qiqihar University, Qiqihar 161006, China |
| Abstract: | Rye and wheat brans are valuable sources of bioactive compounds, which could be used for the development and commercialization of high added value functional ingredients such as dietary antioxidants. The aim of this study was to evaluate antioxidant potential of rye and wheat bran using different polarity solvents. Cereal brans were ground to four different particle size fractions and extracted at 10.3 MPa pressure and 80 °C temperature by consecutive application of hexane, acetone and methanol:water (80:20%). The highest extract yield was obtained from rye bran using methanol-water; particle size in most cases had a significant effect. Antioxidant potential of extracts was assessed by ABTS+•, DPPH• scavenging, ORAC and total phenols content (TPC) assays. Extraction solvent had a major influence on TPC and antioxidant activity of the extracts. The most active extracts were obtained using methanol:water; rye bran extracts, in general, were stronger antioxidants than wheat bran extracts. For the majority of assays, reduction of particle size resulted in higher antioxidant activity values. However, ABTS+• scavenging was found to decrease by decreasing particle size of rye bran used for extraction. |
