| 新疆甜瓜高效再生体系的建立及NPR1/HRP双价抗病基因转化 |
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| 引用本文： | 冯玉杰,王爱英,沈海涛,祝建波.新疆甜瓜高效再生体系的建立及NPR1/HRP双价抗病基因转化[J].北方园艺,2011(3):144-148. |
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| 作者姓名： | 冯玉杰 王爱英 沈海涛 祝建波 |
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| 作者单位： | 石河子大学,农业生物技术重点实验室,新疆,石河子,832000;石河子大学,农业生物技术重点实验室,新疆,石河子,832000;石河子大学,农业生物技术重点实验室,新疆,石河子,832000;石河子大学,农业生物技术重点实验室,新疆,石河子,832000 |
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| 基金项目： | 国家自然科学基金，石河子大学高技术引进人才资助项目 |
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| 摘 要： | 以厚皮甜瓜5d龄无菌苗子叶为外植体,MS和1/2MS为基本培养基,研究了添加6-BA、IAA和卡那霉素不同浓度,进行甜瓜再生体系与根癌农杆菌介导的转化体系的建立。结果表明:甜瓜子叶最适宜的不定芽分化培养基为MS+6-BA 1.0mg/L+IAA 0.3mg/L,芽伸长培养基为MS+6-BA 0.75mg/L+IAA 0.3mg/L,生根培养基为1/2MS+IAA 0.3mg/L,适宜的转化条件为外植体预培养48h,农杆菌菌液浓度为OD600=0.3～0.4,侵染时间为15min,22℃暗培养48～55h。经75mg/L的卡那霉素筛选,共获得再生甜瓜幼苗21株,其中8棵经PCR鉴定证明NPR1/HRP双价抗病基因已经整合到甜瓜基因组中,即转化率为38%。
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| 关 键 词： | 甜瓜 再生体系 双价抗病基因 农杆菌介导 |
Research on Construction of Regeneration System of Xinjiang Muskmelon and Transformation with NPR1 and HRP Disease-resistance Bivalent Gene |
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| FENG Yu-jie,WANG Ai-ying,SHEN Hai-tao,ZHU Jian-bo.Research on Construction of Regeneration System of Xinjiang Muskmelon and Transformation with NPR1 and HRP Disease-resistance Bivalent Gene[J].Northern Horticulture,2011(3):144-148. |
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| Authors: | FENG Yu-jie WANG Ai-ying SHEN Hai-tao ZHU Jian-bo |
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| Institution: | FENG Yu-jie,WANG Ai-ying,SHEN Hai-tao,ZHU Jian-bo (Agriculture Biotechnology of Major Lab of Shihezi University,Shihezi,Xinjiang 832000) |
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| Abstract: | In order to establish the regeneration and transformation system,the cotyledons of five-day muskmelon as explants,MS and half-strength MS medium as basic medium with different 6-BA,IAA and kanamycin.The results showed that the optimum medium for Adventitious buds was MS medium supplemented with 1.0 mg/L 6-BA+0.3 mg/L IAA,the medium for adventitious buds to elongate was MS medium supplemented with 0.75 mg/L 6-BA+0.3 mg/L IAA,the root induction medium was 1/2 MS medium with 0.3 mg/L IAA The optimum transforma... |
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| Keywords: | muskmelon regeneration system disease-resistance bivalent gen agrobacterium-mediated |
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