| 东北山樱根际细菌群落结构多样性分析 |
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| 引用本文： | 秦嗣军,吕德国,李志霞,刘灵芝,刘国成,马怀宇.东北山樱根际细菌群落结构多样性分析[J].吉林农业大学学报,2011,33(6):643-648. |
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| 作者姓名： | 秦嗣军 吕德国 李志霞 刘灵芝 刘国成 马怀宇 |
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| 作者单位： | 1. 沈阳农业大学园艺学院,沈阳,110161
2. 中国农业科学院果树研究所,兴城,125100 |
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| 基金项目： | 国家自然科学基金项目(30871688,30900967); 辽宁省教育厅项目(2008631) |
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| 摘 要： | 利用PCR- DGGE技术研究了野生与栽培两种生境下东北山樱的表层和根际土壤细菌群落结构的差异,以及野生东北山樱根际与其周围优势植物根际细菌群落结构的区别.用细菌的16S rDNA特异引物,对东北山樱和相邻其他优势植物根区土壤中提取的总DNA进行PCR扩增,通过DGGE技术对PCR产物进行分析.结果表明:无论在栽培条件...
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| 关 键 词： | 东北山樱 细菌 群落结构 多样性 |
Analysis of the Bacterial Community Structures Diversity in Rhizosphere of Cerasus sachalinensis Kom. |
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| QIN Si-iun,Lü De-guo,LI Zhi-xia,LIU Ling-zhi,LIU Guo-cheng,MA Huai-yu.Analysis of the Bacterial Community Structures Diversity in Rhizosphere of Cerasus sachalinensis Kom.[J].Journal of Jilin Agricultural University,2011,33(6):643-648. |
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| Authors: | QIN Si-iun Lü De-guo LI Zhi-xia LIU Ling-zhi LIU Guo-cheng MA Huai-yu |
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| Institution: | QIN Si-jun1,Lü De-guo1,LI Zhi-xia2,LIU Ling-zhi1,LIU Guo-cheng1,MA Huai-yu11.College of Horticulture,Shenyang Agricultural University,Shenyang 110161,China,2.Research Institute of Pomology,Chinese Academy of Agricultural Sciences,Xingcheng 125100 |
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| Abstract: | The bacterial community structures of Cerasus sachalinensis Kom.in topsoil and rhizospheric soil in wild and cultivated conditions were studied by means of PCR-DGGE(denaturing gradient gel electrophoresis),and their differences in the rhizosphere of Cerasus sachalinensis and other neighboring plant in the pristine growing conditions were also analyzed.Partial genes were amplified from soil bacterial community DNA using 16S rDNA gene primers.The diversity of PCR-amplified products was detected by DGGE.The re... |
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| Keywords: | Cerasus sachalinensis bacteria community structure diversity |
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