| 利用虫体蛋白特异性区分三种象虫 |
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| 引用本文： | 赵英杰,霍权恭,张来林,周展明,姜永嘉.利用虫体蛋白特异性区分三种象虫[J].粮食储藏,2000,29(1):17-19. |
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| 作者姓名： | 赵英杰 霍权恭 张来林 周展明 姜永嘉 |
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| 作者单位： | 郑州粮食学院,450052 |
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| 摘 要： | 本文报道了利用聚丙烯酰胺凝胶电泳（PAGE）和凝胶渗透液相色谱法（GPC）分别对3种象虫虫体蛋白进行分离分析。初步分析结果表明：凝胶电泳法具有较高的分辨能力，电泳图谱能明显地区分3种象虫；凝胶渗透液相色谱法分析结果无差异，但对3种象虫虫体蛋白的胰蛋白酶水解液的分离结果有明显的区别。
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| 关 键 词： | 米象 玉米象 谷象 虫体蛋白特异性 PAGE GPC |
IDENTIFICATION OF SITOPHILUS SPP.WITH PAGE AND HPLC METHOD |
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| Zhao Yinjie,Huo Quangong,Zhang Lailin,Zhou Zhanming,Jiang Yongjia.IDENTIFICATION OF SITOPHILUS SPP.WITH PAGE AND HPLC METHOD[J].Grain Storage,2000,29(1):17-19. |
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| Authors: | Zhao Yinjie Huo Quangong Zhang Lailin Zhou Zhanming Jiang Yongjia |
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| Institution: | Zhengzhou Grain College |
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| Abstract: | The identification of Sitophilus spp. was studied with PAGE method and HPLC methodto analyze ptoteins of insect. There were significant differences in electrophoretic patters ofurea soluted protein among Sitophilus zeamais, S. oryzae and S. granarius at the conditionof pH9. 1 and gel concentration of 7. 5 and 10 % in PAGE. Sitophilus zeamais and S. oryzaecould also be distinguished in HPLC of enzyme change protein. The two methods can beapplied to identification of Sitophilus spp.. |
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| Keywords: | Sitophilus oryzae S zeamais S granarius identification electrophoresis LC |
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