| 猪DECR1基因SNPs筛选及其多态性分析 |
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| 引用本文： | 黄生强,柳小春,蒋隽,马乐.猪DECR1基因SNPs筛选及其多态性分析[J].湖南农业大学学报(自然科学版),2011,37(2):192-194. |
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| 作者姓名： | 黄生强 柳小春 蒋隽 马乐 |
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| 作者单位： | 湖南农业大学动物科学技术学院,湖南长沙,410128 |
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| 基金项目： | 国家"863"项目，湖南省教育厅项目 |
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| 摘 要： | 以大白猪、长白猪和杜洛克3个猪种共327头为试验材料,对2,4-dienoyl-CoA reductase 1(DECR1)基因第6至第10外显子区域进行SNPs筛选,分析SNP突变位点的遗传多态性;采用比较基因组方法,根据人DECR1基因全长序列设计引物,筛选猪DECR1基因的SNP,经PCR-SSCP技术和测序检测...
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| 关 键 词： | 猪 单核苷酸多态性 DECR1基因 遗传多态性 |
| 收稿时间： | 2010/12/20 0:00:00 |
| 修稿时间： | 2011/3/8 0:00:00 |
SNPs of DECR1 gene and its genetic polymorphism in pigs |
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| huang sheng qiang,liu xiao chun,jiang juan and ma le.SNPs of DECR1 gene and its genetic polymorphism in pigs[J].Journal of Hunan Agricultural University,2011,37(2):192-194. |
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| Authors: | huang sheng qiang liu xiao chun jiang juan and ma le |
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| Institution: | College of Animal Science and Technology;HNAU,College of Animal Science and Technology;HNAU,College of Animal Science and Technology;HNAU,College of Animal Science and Technology;HNAU |
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| Abstract: | Three hundred and twenty seven Yorkshire, Landrace and Duroc pigs were analysed to screen the SNPs in 610 Exon of mitochondrial 2,4-dienoyl-CoA reductase 1(DECR1) gene and analyse its genetic structure. The resluts showed: the primers were designed accroding to human DECR1 gene by means of genome comparison, 2 SNPs were screened out in Exon6 and Exon8 by PCR-SSCP method, but mutation in Exon6 loic belonged to nonsense mutation, mutation in Exon8 loci belong to missense mutation. The polymorphism of the SNP in Exon8 were studied and 3 genotypes (CC, CG, GG) were tested in 3 breeds. There were abundant genetic polymorphism in Exon8 loci. |
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| Keywords: | pig single nucleotide polymorphisms(SNP) DECR1 gene genetic polymorphism |
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