| 八仙花组织培养繁殖技术 |
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| 引用本文： | 冯润东,孙宏刚.八仙花组织培养繁殖技术[J].吉林林学院学报,2011(3):350-352. |
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| 作者姓名： | 冯润东 孙宏刚 |
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| 作者单位： | [1]吉林市林业科学研究院,吉林吉林132013 [2]吉林省林业勘察设计研究院,吉林长春130022 |
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| 摘 要： | 以八仙花的茎段、茎尖、叶片为外植体,在MS培养基中加入不同质量分数的6-苄基腺嘌呤（6-BA）和吲哚丁酸（IBA）进行增殖培养.结果表明：适宜八仙花外植体表面灭菌的方法是利用0.1%氯化汞（HgC l2）灭菌7 m in;不同类型的八仙花外植体在初代培养时的增殖效果不同,茎尖在培养过程中首先伸长生长,然后陆续长出侧芽,表明八仙花的茎尖是比较适合用来进行增殖培养的外植体;继代培养以MS＋6-BA 1.0 mg.L-1＋IBA0.12 mg.L-1为较适宜的增殖培养基;侧芽在1/2 MS＋IBA 0.2 mg.L-1培养基中生根率和单苗根数量均较高,说明1/2 MS＋IBA 0.2 mg.L-1为较好的不定根诱导培养基.
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| 关 键 词： | 八仙花 组织培养 繁殖 |
Propagation Technology of Tissue Culture of Hydrangea macrophylla |
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| Authors: | FENG Run-dong SUN Hong-gang |
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| Institution: | 1.Academy of Forestry Science of Jilin City,Jilin 132013,China;2.Forestry Reconnoitre and Design Research Instidute of Jilin Province,Changchun 130022,China) |
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| Abstract: | Hydrangea macrophylla propagation were studied stem or leaf in culture medium with different 6-BA or IBA.The result showed：the good method for surface sterilization of explant was soaking them in 0.1% HgCl2 for 7min.Propagation was different because of explant types.Bud stem was good explant for propagation because it grows first and regenerates lateral bud.The propagation medium of Hydrangea macrophylla was MS＋6-BA 1.0 mg·L-1 ＋ IBA 0.12 mg·L-1.There were higher rooting rate and more adventitious roots in 1/2 MS＋IBA 0.2 mg·L-1.It was good medium to induce adventitious root of Hydrangea macrophylla. |
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| Keywords: | Hydrangea macrophylla tissue culture propagation |
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