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| 引进国外蓝莓品种的组织培养及快繁技术研究 | |
| 引用本文： | 茨韦特科夫·约旦,赵燕丽,王春,董宣,杨帆.引进国外蓝莓品种的组织培养及快繁技术研究[J].黑龙江农业科学,2011(6):6-9. |
| 作者姓名： | 茨韦特科夫·约旦 赵燕丽 王春 董宣 杨帆 |
| 作者单位： | 1. 黑龙江省农业科学院对俄中心,黑龙江哈尔滨,150086 2. 哈尔滨市职工医学院,黑龙江哈尔滨,150020 3. 黑龙江省农业科学院植物保护研究所,黑龙江哈尔滨,150086 4. 哈尔滨科瑞种苗生物技术研究所,黑龙江哈尔滨,150086 |
| 基金项目： | 哈尔滨市科技攻关计划资助项目 |
| 摘 要： | 以从保加利亚引进的蓝莓品种B91和B92的未萌动枝条为试验材料,进行了顶芽、茎尖和茎段的萌发与分化、茎段的继代丛生与快繁、试管苗生根与移栽等技术的研究。结果表明：对未萌动枝条进行预处理能明显提高接种效率;WPM＋ZEA 2.0 mg.L-1是接种茎段的适宜培养基,WPM＋ZEA 1.0 mg.L-1是大量产生丛生芽的快繁培养基,1/2 WPM＋IBA 2.0 mg.L-1＋NAA 0.5 mg.L-1是生根的理想培养基;在温室中草炭、河砂和园土按1∶1∶1混合,经过硫粉调酸后是试管苗移栽的较好基质;移植到示范果园的试管苗长势旺盛。 |
| 关 键 词： | 蓝莓 组织培养 快繁技术 硫 |
Study on the Techniques of Tissue Culture and Rapid Propagation of Introduced Foreign Blueberry Varieties |
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| Tsvetkov Yordan,ZHAO Yan-li,WANG Chun,DONG Xuan,YANG Fan.Study on the Techniques of Tissue Culture and Rapid Propagation of Introduced Foreign Blueberry Varieties[J].Heilongjiang Agricultural Science,2011(6):6-9. | |
| Authors: | Tsvetkov Yordan ZHAO Yan-li WANG Chun DONG Xuan YANG Fan |
| Institution: | 1.Heilongjiang Center for Agricultural Scientific and Technological Cooperation between China and Russia,Heilongjiang Academy of Agricultural Sciences,Harbin,Heilongjiang 150086;2.Harbin Staff and Workers Medical College,Harbin,Heilongjiang 150020;3.Plant Protection Institute of Heilongjiang Academy of Agricultural Sciences,Harbin,Heilongjiang 150086;4.Harbin Kerui Seed and Seedlings Biotechnology Research Institute,Harbin,Heilongjiang 150086) |
| Abstract: | Dormant shoot of introduced blueberry varieties of B91 and B92 from Bulgaria were used as experimental materials to carry out a study on techniques of budding and dissimilating of apical bud,stem apex and stem segments,regeneration and fast propagation of stem segments,rooting and transplanting of seedlings in test tube.The results showed that the pre-treatment on dormant shoot has significantly increased efficiency of inoculation;WPM＋ZEA 2.0 mg·L-1 was a suitable medium for stem segment inoculation;WPM＋ZEA 1.0 mg·L-1 was a fast propagation medium producing a large number of clusters;1/2 WPM＋IBA 1.0 mg·L-1＋NAA 0.5 mg·L-1 was the ideal medium for rooting.In the greenhouse peat,river sand and garden soil mixed at 1∶1∶1 ratio,and treated with sulphur powder after acid was a comparatively good substrate for transplantation of seedlings produced in test tube;Transplanted seedlings performed vigorous growth in the demonstrate orchard. |
| Keywords: | blueberry tissue culture fast propagation techniques |
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