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| 复合诱变选育柚苷酶高产菌株的研究 | |
| 引用本文： | 李志坚,谭兴和,单杨,李高阳.复合诱变选育柚苷酶高产菌株的研究[J].湖南农业科学,2010(3):1-3,7. |
| 作者姓名： | 李志坚 谭兴和 单杨 李高阳 |
| 作者单位： | 1. 湖南省农产品加工研究所,湖南,长沙,410125;湖南农业大学食品科技学院,湖南,长沙,410128 2. 湖南农业大学食品科技学院,湖南,长沙,410128 3. 湖南省农产品加工研究所,湖南,长沙,410125 |
| 基金项目： | 国家"863"计划项目 |
| 摘 要： | 以柚苷酶产生菌黑曲霉FY01—4为试验菌株，采用紫外线和60Co-γ射线进行复合诱变，经摇瓶复筛选育出一株柚苷酶高产菌株C15，酶活达1285．6U／mL，是出发菌株的3．59倍。连续传代10代，酶活仍保持在1200～1300U／mL之间，菌株产酶稳定性良好，可作为生产柚苷酶的优良菌株。 |
| 关 键 词： | 复合诱变 柚苷酶 紫外线 60 Co—γ射线 选育 |
Selective Breeding High-producing Strain of Naringinase by Complex Mutagenesis |
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| LI Zhi-jian,TAN Xing-he,SHAN Yang,LI Gao-yang.Selective Breeding High-producing Strain of Naringinase by Complex Mutagenesis[J].Hunan Agricultural Sciences,2010(3):1-3,7. | |
| Authors: | LI Zhi-jian TAN Xing-he SHAN Yang LI Gao-yang |
| Abstract: | A producing strain of naringinase -Aspergillus niger FY01-4 was taken as experimental strain, using ultraviolet ray and 60 Co-γray to complex mutagenize it. Then, a high-producing strain of naringinase C15 was secondarily screened with shake bottle, whose enzyme activity reached 1 285.6 U/mL and it was 3.59 times higher than that of original strain. Serial passage 10 generations, the enzyme activity of C15 kept in a range of 1 200 U/mL to 1 300 U/mL, the stability of producing strain was great, it could be a choiceness strain to produce naringinase. |
| Keywords: | 60 complex mutagenesis naringinase ultraviolet ray selective breeding |
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