猪GH基因全序列PCR-RFLPs研究

实验利用PCR-RFLPs技术分析了大约克猪和长白猪GH基因的BstⅪ和HhaⅠ两种内切酶酶切位点多态性。结果表明,在所检约克夏猪和长白猪两个品种中,pGH基因全序列内仅有1个BstⅪ酶切位点,未发现BstⅪ酶切位点多态性;pGH基因全序列中存在丰富的HhaⅠ酶切位点多态性,约克夏猪中有10种带型,长白猪中检出了8种带型,其中B带型频率最高,长白猪中为41 67%,约克夏猪中占31 11%,二者差异极显著(P<0 01)。其他各带型频率分布在两品种间差异不显著。

Analysis of PCR-RFLPs in Porcine Growth Hormone Gene Full-length

BstⅪ and HhaⅠ polymorphism of pGH gene's full sequence by PCR-RFLP in Yorkshire and Landrace is analyzed. The results of RFLPs analysis indicate that there is only one BstⅪ restriction enzyme site, without discovering BstⅪ restriction enzyme site polymorphism in the pGH gene full length, but there are abundant HhaⅠ restriction enzyme site polymorphisms. After digesting with HhaⅠ restriction enzyme, the amplified 2107?bp DNA segments show 5～9 restriction enzyme sites and 6～10 fragments. A～J ten band-types in Yorkshire pigs and eight band-types in Landrace pigs can be distinguished, and F and I band-types in Landrace pigs are not discovered. The frequency of B band-type is the highest; it is 31.11% in Yorkshire and 41.67% in Landrace, the difference is the most significant (P<0.01) between Yorkshire and Landrace. However, the distribution differences of other band-types are not significant (P>0.05) between two breeds pigs.