一种蚜虫持久性传病的长豇豆黄化型病毒

 1983年6月,在南京郊区的长豇豆上采到1株表现植株矮缩症状的C-7病毒分离物。接种试验证明,它不能摩擦接种传病,但可以由豆蚜(Aphis craccivora)、棉蚜(A.gossypii)和桃蚜(Myzus persicae)以持久性方式传病。寄主范围测定的结果表明:分离物可以侵染长豇豆、豇豆、蚕豆、大豆、菜豆、豌豆、赤豆、利马豆、苜蓿、红三叶、地三叶、绛三叶、葫芦巴,紫云英和苕子等15种豆科植物和曼陀罗1种茄科植物。这些植物大都出现植株矮化,叶片扭曲,卷缩或僵缩,不能开花结实等症状。豆蚜的传病性状中,获毒饲育的最短传病时间为3小时,接毒饲育最短传病时间为10分钟,循回期是24小时左右。但是,传病率最高的获毒饲育时间是2~3天,接毒饲育时间在1天以上。接种1头蚜虫就具有传病能力,5头蚜虫能达到100%的传病率。蚜虫可以终身传毒,蜕皮不影响其传毒力,但传毒有间歇性。根据它的基本性状,病毒C-7分离物是一种豆科植物的黄化型病毒,可能是属于大麦黄矮病毒组(Luteovirus Group)的成员。

A PERSISTENT APHID-TRANSMITTED VIRUS OF ASPARAGUS BEAN(VIGNA SESQUIPEDALIS) IN NANJING

A virus disease of asparagus bean characterized by a dwarfing of plant and curling or rugosity of leaves,has been found in Nanjing area in 1983.The isolate C-7 of this virus was transmitted by three species of aphids,efficiently by Aphis craccivora and less efficiently by Aphis gossypii and Myzus persicae in the persistent manner,but not by Aphis glycine.The C-7 isolate was not transmitted by sap inoculation.Of 28species of plant in 4 families which were inoculated by the aphids(A.craccivora),15 leguminous species and 1 solanaceous species were found to be infected with the virus.The aphid was able to acquire the virus by feeding on the infected asparagus bean plant for at least 3 hours and the inoculation feeding period was at least 10 minutes.The latent period in the aphid vector was 24-36 hours.In serial transmission tests,individual aphids seemed to transmit the virus as long as they livedand retained their infectivity through molting,although not every plant in a series was infected.Based on symptoms,host range and vector transmission,the virus can be considered as a persistent aphid-tran-smitted virus belonging to the group of Luteoviruses.