珙桐花粉离体萌发及花粉管生长研究

[目的]揭示珙桐濒危的原因和机制,为珙桐花粉保存和杂交育种工作及保护提供参考。[方法]运用方差分析、多重比较等方法对珙桐花粉的萌发和花粉管生长进行了研究。[结果]研究得出,培养基内蔗糖、硼酸、琼脂在一定浓度范围内,对花粉萌发及花粉管生长起促进作用,但超过一定浓度时则起抑制作用;吲哚乙酸对花粉萌发及花粉管生长影响不显著。温度20~25℃时,珙桐花粉萌发和花粉管生长最好;同时,花粉萌发也要求合适的湿度条件,湿度太低花粉萌发率低,湿度太大花粉管易吸水破裂。[结论]珙桐花粉萌发和花粉管的生长因培养基组分和培养条件的的改变而不同,其花粉萌发和花粉管生长不仅需要一个适宜的组分浓度,也需要有一个合适的培养条件。

Research on the Pollen Germination and Growth in vitro of Davidia involucrata

[Objective] The causes and mechanism for Davidia involucrata endangered were revealed so as to provide the reference for its pollen preservation and cross breeding.[Method]The pollen germination and pollen tube growth of Davidia involucrata were tested with one way ANOVA and multiple-comparison.[Results] The results indicated that the pollen germination and pollen tube growth would be enhanced when the concentration of sucrose,boric scid,agar in the culture medium were within a certain range.If the concentrations were too high or low,the pollen germination and pollen tube growth would be inhibited.The effect of IAA on pollen germination and pollen tube growth was in significant.The optimum temperature affecting pollen germination and pollen tube was from 20 to 25 ℃.Humidity had big effect on pollen fertility.When atmosphere was dry,its germination ability was reduced.But when atmosphere was too humid,the pollen drank too much water and broke,and its germination ability was reduced,too.[Conclusion] Davidia involucrata pollen germination and pollen tube growth varied with the change of medium component and culture conditions and its pollen germination and pollen tube growth required not only an appropriate concentration of the components,but also had the suitable culture condition.