Isolation of <Emphasis Type="Italic">Brucella melitensis</Emphasis> from a RB51-vaccinated seronegative goat |
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Authors: | Enrique Herrera Aldo Rivera E Gabriela Palomares Rigoberto Hernández-Castro Efrén Díaz-Aparicio |
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Institution: | Department of Bacteriology, CENID Microbiología, Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias, Carretera Federal México-Toluca Km. 15.5, Cuajimalpa, México City, CP, 05110, Mexico. |
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Abstract: | The purpose of this study is to determine the etiology of abortions presented in a goat herd declared as free of brucellosis
and vaccinated with RB51 located in Mexico. The serological diagnosis of brucellosis in 33 animals was performed. The study
included three goats that aborted in the last third of gestation and 15 goats that gave birth normally; samples of milk and
vaginal exudate were subjected to bacteriological study. All animals were negative for serological diagnosis, and isolation
of Brucella melitensis was achieved in a single goat from vaginal exudate. However, the particularity is that this goat was negative to the card,
indirect ELISA, and radial immunodiffusion tests. Isolation of a field strain was confirmed by biochemical test resistance
to rifampicin and PCR. It is concluded that a goat which aborted in the last third of gestation was found spreading B. melitensis through vaginal discharge despite being vaccinated with RB51 and seronegative for brucellosis. |
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