12个金针菇菌株菌丝的生长速度及胞外酶活力比较

主要比较了生产上常用的12个金针菇Flammulina velutiper(Fr.)Sing.]菌株菌丝的生长速度及菌落特征,同时,对12个菌株的胞外酶,如羧甲基纤维素酶、木聚糖酶、漆酶的活力进行了测定,以期找出菌丝生长速度与胞外酶活力的相关性,为正确评价菌种质量提供科学依据。结果表明,12个菌株间菌丝生长速度存在差异,在PDA加富培养基上生长较快的菌株是9号和12号;在模拟出菇培养基上生长较快的菌株是7号、8号和12号。羧甲基纤维素酶活力最高的菌株是5号,木聚糖酶和漆酶活力最高的菌株是1号。胞外酶活力大小表现的顺序与生长速度偶联性较差。

Study on the Hypha Growth Speed and Extracellular Enzymes Activity of 12 Strains of Flammulina velutiper

The hypha growth speed and colony characteristics of 12 stains of Flammulina velutiper (Fr.) Sing commonly used on the production was compared.Moreover,the activity of their extracellular enzymes,such as Carboxymethyl cellulose,xylanase,laccase enzyme were determined in order to find out the relationship between hypha growth rate and extracellular enzyme activity so as to provide scientific basis for evaluation of strain quality.The experimental results show that the growth speed of strain No.9 and No.12 was faster than the others on PDA medium with rich inorganic salts and vitamin;while the growth speed of strain No.7,No.8,and No.12 was faster than others on simulated mushroom medium.Carboxymethyl cellulase is the most active in strain No.5;while xylanase and laccase is the most active in strain No.1.The contingency between extracellular enzyme activity and speed coupling is poorer.