蒲公英的组织培养研究

以盆栽蒲公英的叶柄和叶片为试材，对蒲公英进行组织培养。结果表明：300mg/L的抗坏血酸能够较好地防止褐化，直接诱导再生植株的最佳组合是MS＋0.5mg/L6-BA＋0.1mg/LNAA；诱导愈伤组织和继代的最佳组合是6-BA0.5mg/L＋2,4-D0.5～1.0mg/L；1/2MS＋15g/L蔗糖＋NAA0.1mg/L是诱导生根的理想培养基。

Tissue Culture of Dandeli

With pot-cultured dandelion as test material, the leaf stalk and blade were used as explants for tissue culture. Addition of 300 mg/l of vitamin C into the culture medium was most effective to prevent callus from browning, and the medium MS + 0.5 mg/l 6-BA + 0.1 mg/l NAA is an optimum combination for direct regeneration. For indirect regeneration fragile calli were obtained from explants on the medium supplemented with 6-BA 0.5 mg/l and 2,4-D 0.5~1.0 mg/l. The medium 1/2 MS + NAA 0.1 mg/l was suitable for rooting.