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| 文心兰的组织培养和移栽管理技术 | |
| 引用本文： | 邓小果,黎维诗,柯海丽,凌绪柏,刘亚文,林木龙. 文心兰的组织培养和移栽管理技术[J]. 热带作物学报, 2010, 30(7): 48-51 |
| 作者姓名： | 邓小果 黎维诗 柯海丽 凌绪柏 刘亚文 林木龙 |
| 作者单位： | 海南博大兰花科技有限公司 海南海口 570311;海南博大兰花科技有限公司 海南海口 570311;海南博大兰花科技有限公司 海南海口 570311;海南博大兰花科技有限公司 海南海口 570311;海南博大兰花科技有限公司 海南海口 570311;海南博大兰花科技有限公司 海南海口 570311 |
| 摘 要： | 以文心兰的侧芽为外植体，对侧芽的选择、处理，外植体的消毒方法、培养基的选择等技术进行研究。结果表明：最适合的材料为叶片还未展开的侧芽，二次消毒对文心兰侧芽消毒效果最好。文心兰理想的诱导培养基为MS＋6－BA 5.0 mg/L＋NAA 0.5 mg/L＋椰子水100 mL/L，增殖培养基为MS＋6－BA 2.0 mg/L＋NAA 0.2mg/L+椰子水100 mL/L，壮苗培养基为MS＋香蕉20g/L，生根培养基为1/2MS＋NAA 1.0 mg/L+香蕉50 g＋土豆20 g/L＋AC2.0 g/L。同时 |
| 关 键 词： | 文心兰 ；组织培养 ；移栽 |
Tissue Culture and Transplanting and Management of Oncidium |
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| Affiliation: | Hainan Boda Orchid Technology Co. LTD, Haikou, Hainan 570311;Hainan Boda Orchid Technology Co. LTD, Haikou, Hainan 570311;Hainan Boda Orchid Technology Co. LTD, Haikou, Hainan 570311;Hainan Boda Orchid Technology Co. LTD, Haikou, Hainan 570311;Hainan Boda Orchid Technology Co. LTD, Haikou, Hainan 570311;Hainan Boda Orchid Technology Co. LTD, Haikou, Hainan 570311 |
| Abstract: | The lateral buds of oncidium were used as explants. The effects of material, disinfecting methods and culture media on in vitro growth of oncidium were investigated. The results were as follows: the best material was the lateral buds whose blades did not unfold. Two times of consecutive disinfecting attained the best result. The most appropriate culture media were as follows: induction medium MS+6-BA 5.0 mg/L+NAA 0.5 mg/L+coconut water 100 mL/L; proliferation medium MS+6-BA 2.0 mg/L+NAA 0.2 mg/L+ coconut water 100 mL/L; seedling medium MS+banana 20 g/L; rooting medium 1/2MS+NAA 1.0 mg/L+ bananas 50 g/L+potato 20 g/L+AC 2.0 g/L. The transplanting survival rate was over 90% after pretreatment and management of oncidium plantlets. |
| Keywords: | Oncidium tissue culture transplanting |