OT型百合品种黄天霸花器官组培快繁体系的建立

[目的]研究OT型百合品种黄天霸花器官的离体培养快速繁殖技术,为其种球产业化生产提供技术支持.[方法]以黄天霸花蕾和半开花的不同部位为外植体,以MS为基本培养基,探讨不同外植体的愈伤组织诱导效果、0～3.0 mg/L6-BA对外植体愈伤组织分化、1.0～3.0 mg/L 6-BA＋0～0.2 mg/L NAA组合对叶片不定芽诱导、30.0～80.0 mg/L蔗糖对鳞茎形成及生根的影响,调查不同基质对组培苗移栽种植的效果.[结果]花蕾和半开花不同部位愈伤组织诱导从易到难表现为：子房＞柱头＞花药＞花托＞花丝、花瓣,子房和柱头的愈伤组织诱导率均超过50.0％.在MS培养基中,随着6-BA用量的增加（0～3.0 mg/L）,外植体愈伤组织分化率先提高后稍有下降,适宜分化培养基为MS＋ 2.5 mg/L 6-BA.在不同6-BA和NAA组合中,以培养基MS＋3.0 mg/L 6-BA的无菌叶片不定芽诱导效果较优.添加60.0 g/L蔗糖的MS培养基利于不定芽结鳞茎生根;带鳞茎组培苗移栽于大田菜园土即可成活.[结论]成功建立的OT型百合品种黄天霸花器官离体快速繁殖体系可用于其种球工厂化生产,扩大规模化种植.

Tissue culture and rapid propagation of OT hybrids lily Manissa floral organs

[Objective]The in vitro culture and rapid propagation of floral organs derived from OT hybrids lily Manissa was conducted to provide technical supports on industrial production of its seed-bulbs.[Method]Different parts of flower buds and half-open flower of Manissa were used as explants and MS was used as base culture medium.The callus induction effects of different explants were investigated.The influences of 0-3.0 mg/L 6-BA on callus differentiation culture of explants were also analyzed,as well as different combinations of 1.0-3.0 mg/L 6-BA and 0-0.2 mg/L NAA on adventitious buds induction derived from sterile leaves,30.0-80.0 mg/L sugar on bulblet formation and rooting culture.Furthermore,different planting substrates were used for plantlet transplanting.[Result] The different parts of flower buds and half-open flower presented different callus induction effects,the ovary showed the easiest in callus induction,followed by stigma,anther,receptacle,filament and petal.The callus induction rates of both ovary and stigma was over 50.0％.In MS medium,the differentiation rate of callus showed increase firstly and then decrease with the increase of 6-BA（0-3.0 mg/L）.The suitable callus differentiation medium was MS＋2.5 mg/L 6-BA.By adding different combinations of 6-BA and NAA in MS,it found that MS＋3.0 mg/L 6-BA was appropriate for induction of adventitious buds derived from sterile leaves.Adding 60.0 g/L sugar in MS medium could promote bulblet formation and rooting.The induced plantlets with bulblets could survive by transplanting to vegetable garden soil.[Conclusion]The in vitro rapid propagation system was established for floral organs at different stages of OT hybrids lily Manissa,which could be applied on factory production of Manissa seed-bulbs to extend largescale cultivation in Guangxi.