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不同因素对香石竹初代培养的影响
引用本文:李军,徐虹,王晓敏.不同因素对香石竹初代培养的影响[J].安徽农学通报,2008,14(19):41-42.
作者姓名:李军  徐虹  王晓敏
作者单位:1. 宁夏职业技术学院生物工程系,宁夏银川,750002
2. 西北农林科技大学生命科学学院,陕西杨凌,712100
3. 西北农林科技大学植物保护学院,陕西杨凌,712100
摘    要:研究了不同因素对香石竹初代培养的影响,结果表明:用75%酒精灭菌1min,再用0.1%HgCl2灭菌20 min后,香石竹外植体的成活率最高;以4号培养基对叶片进行愈伤组织诱导,其诱导率最高,达到了95%;以黄色香石竹叶片为外植体进行初代培养,诱导率最高,为88%。

关 键 词:香石竹  叶片  初代培养  培养基

Effect of Different Factors on Primary Culture of Carnation
LI Jun et al..Effect of Different Factors on Primary Culture of Carnation[J].Auhui Agricultural Science Bulletin,2008,14(19):41-42.
Authors:LI Jun
Institution:LI Jun1 et al.
Abstract:The leaves of carnation were used as explants and effects the different factors on primary culture of carnation were studied.The results showed that 1 min with 75% ethyl alcohol and 20 min with 0.1% HgCl2 were the best method to reduce pollutions,and the carnation explants survival percentage was higher;the No.4 medium was used to induce callus tissue,the inductivity was the highest and up to 95%;the yellow leaves of carnation were used as explants and primarily cultured,the inductivity was the highest and up to 88%.
Keywords:Carnation  leaves  Primary culture  medium
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