国兰“绿蕙红舌”的组织培养技术

采用不同培养基对国兰"绿蕙红舌"的种子进行组织培养试验研究,综合分析不同培养基配方在国兰"绿蕙红舌"组织培养过程中对种子萌发与原球茎诱导、原球茎继代增值、原球茎分化及生根壮苗的影响。结果表明,种子萌发与原球茎诱导最适宜培养基为MS+BA 0.5 mg·L-1+NAA 0.5 mg·L-1+水解酪蛋白100 mg·L-1+马铃薯汁200 mg·L-1+蔗糖30 g·L-1+琼脂4 g·L-1;原球茎继代增值最适培养基为KC+BA0.5 mg·L-1+NAA 0.5 mg·L-1+活性碳2g·L-1+马铃薯汁200 mg·L-1+蔗糖30 g·L-1+琼脂4 g·L-1;原球茎分化最适培养基为KC+BA1.0 mg·L-1L+NAA 0.2mg·L-1+活性碳2 g·L-1+马铃薯汁200 mg·L-1+蔗糖30 g·L-1+琼脂4 g·L-1;生根壮苗最适培养基为1/2 MS+NAA 0.5mg·L-1+活性碳2 g·L-1+马铃薯汁200 mg·L-1+蔗糖30 g·L-1+琼脂4 g·L-1。以上培养基pH均为5.1～5.4。

Tissue culture of variety of "Green Wai Red tongue" of Cymbidium

In this paper,different mediums were used to study the tissue culture of Cymbidium"Green Wai Red tongue"from seeds,and the effects on seed germination and protocorm induction,protocorm subculture,protocorm differentiation and rooting were comprehensively analyzed.The results are as follows.The most ap-propriate medium for seed germination and protocorm induction is MS medium supplemented with 0.5 mg?L-1 BA,0.5 mg?L-1 NAA,100 mg?L-1 casein hydrolyzate,200 mg?L-1 potato juice,30 g?L-1 sucrose and 4 g?L-1 agar;for protocorm subculture is KC supplemented with 0.5 mg?L-1 BA,0.5 mg?L-1 NAA,2 g?L-1 activated carbon,200 mg?L-1 potato juice,30 g?L-1 sucrose and 4 g?L-1 agar;for protocorm differentiation is KC medium supplemented with 1.0 mg?L-1 BA,0.2 mg?L-1 NAA,2 g?L-1 activated carbon,200 mL?L-1 potato juice,30 g?L-1 sucrose and 4 g?L-1 agar;and for rooting is 1/2 MS medium supplemented with 0.5 mg?L-1 NAA,2 g?L-1 activated carbon,200 mg?L-1 potato juice,30 g?L-1 sucrose,and 4 g?L-1 agar.pH values of all the mediums are from 5.1 to 5.4.