| 鸭疫里默氏杆菌原生质体的制备研究 |
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| 引用本文： | 王勇,马跃,赵秦,王秋菊,万向.鸭疫里默氏杆菌原生质体的制备研究[J].现代畜牧兽医,2009(1). |
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| 作者姓名： | 王勇 马跃 赵秦 王秋菊 万向 |
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| 作者单位： | 西南大学荣昌校区动物医学系,重庆,402460 |
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| 摘 要： | 鸭疫里默氏秆菌是一种非常难以转化的细菌,国内外未见关于其质粒转化的报道,试验以鸭疫里默氏杆菌OD600为0.7时为受体菌,在高渗缓冲液中,经溶菌酶5 mg/ml于37℃作用50 min,制备的原生质体再生于再生双层固体培养基,原生质体成功再生.研究为鸭疫里默氏杆菌的质粒转化提供了一条新途径.
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| 关 键 词： | 鸭疫里默氏杆菌 原生质体 溶菌酶 再生 |
Generation of Riemerella anatipestifer protoplast |
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| Wang Yong,Ma Yue,Zhao Qin,Wang Qiuju,Wan Xiang.Generation of Riemerella anatipestifer protoplast[J].Modern JOurnal of Animal Husbandry and Veterinary Medicine,2009(1). |
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| Authors: | Wang Yong Ma Yue Zhao Qin Wang Qiuju Wan Xiang |
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| Institution: | Veterinary Department;Southwest University;Chongqing 402460 |
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| Abstract: | When Riemerella anatipestifer OD600=0.7,Riemerella anatipestifer was enzymolysised by lysozyme(5 mg/ml)in order to prepare protoplast.Riemerella anatipestifer protoplast were successfully generated and regenerated on double-deck solid medium.This study offer a new way for the Riemerella anatipestifer plasmid transformation. |
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| Keywords: | Riemerella anatipestifer protoplast lysozyme regeneration |
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